Industry: Biological - Chromatography Online
Industry: Biological

The Early Development of Size-Exclusion Chromatography: A Historical Perspective

July 1, 2013

This column installment traces the early development of SEC from its inception to its acceptance as a modern analytical technique.

HPLC Analysis of Very Polar Compounds in Bioanalysis

June 1, 2013

Joe Pesek answers questions about IEX, HILIC, polar-embedded, and polar-endcapped columns, and aqueous normal phase chromatography.

Analytical Tools for Enabling Process Analytical Technology: HPLC and Quantitative Polymerase Chain Reaction

January 1, 2013

How to use these tools to develop an efficient and robust process

The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part II: Examples and Comparison to Mass Spectrometry

December 1, 2012

Detailed examples of light-scattering techniques are presented as well as how their performance compares to MS and other methods.

Fast Online SPE Purification of Stevia Plant Extracts

September 6, 2012

Sample clean-up using solid-phase extraction (SPE) and similar methods can sometimes be both time-consuming and expensive. The purification of complex plant extracts requires special care in particular. A robust and sensitive online SPE sample preparation method is described for the determination of steviol glycosides.

Asymmetric Flow Field Flow Fractionation Coupled with ICP-MS for Speciation Analysis of Plasma Proteins

August 2, 2012

This work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin: the two main proteins that have been proposed to bind and transport metal ions in this biological fluid.

Plates Versus Selectivity: An Emerging Issue With Complex Samples

August 1, 2012

Smaller particles, increasingly clever ways to overcome mass transfer limitations, further reductions in plate height, shorter separation times, higher pressure, and enhanced peak capacity dominate thinking in the high performance liquid chromatography (HPLC) community today. For healthcare applications, however, we need to think differently about how to improve analytical separations.

An Age of Discovery

July 4, 2012

The Column spoke to Martin Giera of Leiden University Medical Centre, Leiden, The Netherlands, about developments in biomarker discovery.

An Easy Way to a Fast Universal Method for Surfactant Analysis

July 4, 2012

Surfactants present a real challenge to the analytical chemist as they are a very complex class of chemical compounds.


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Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: When Bad Things Happen to Good Food: Applications of HPLC to Detect Food Adulteration

Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: HPLC for Characterization and Quality Control of Therapeutic Monoclonal Antibodies

MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis

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