LC and HPLC - peer-reviewed on research on high performance liquid chromatography - Chromatography Online
LC/HPLC
Peer-reviewed research on HPLC - high performance liquid chromatography. Analytical chemistry info for separation scientists & chromatographers - from LCGC.

Strategies for Rapid Chiral HPLC Method Development for Pharmaceutical Analysis

April 19, 2012

Automated column and mobile phase screening is the current trend in the pharmaceutical industry to develop chiral methods. This study presents comprehensive strategies for automated rapid chiral HPLC method development including normal-phase, reversed-phase and polar organic phase separation modes.

Advancements in the Understanding of Stationary Phases for HILIC

April 1, 2012

Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized separations are described.

A New Generation of Silica-Based Monolithis HPLC Columns with Improved Performance

April 1, 2012

Monolithic columns feature both high column efficiency and high column permeability. This article discusses their preparation, applications, and future prospects.

Determination of Phenylurea Herbicides in Tap Water and Soft Drink Samples by HPLC–UV and Solid-Phase Extraction

March 1, 2012

A simple and sensitive high performance liquid chromatography (HPLC) method with ultraviolet (UV) detection was developed to analyse phenylurea herbicides: – monuron, diuron, linuron, metazachlor and metoxuron – in three soft drink brands and tap water.

Using MS Data for Peak Tracking in Computer Supported HPLC Method Development

February 1, 2012

A systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms.

A Case of Sporadic LC Assay Results

February 1, 2012

A mysterious autosampler problem is solved.

Current Trends in HPLC Column Technology

January 1, 2012

The results of a recent survey on high performance liquid chromatography (HPLC) columns shows some interesting changes.

Hollow-Fiber Liquid-Phase Microextraction in the Three-Phase Mode – Practical Considerations

December 1, 2011

The practical aspects of hollow-fiber liquid-phase microextraction in the three-phase mode (HF3LPME) are described.

Highlights of HPLC 2011

September 1, 2011

A review of HPLC 2011, which took place in Budapest, Hungary in June this year.

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