Peer-reviewed research on HPLC - high performance liquid chromatography. Analytical chemistry info for separation scientists & chromatographers - from LCGC.
May 1, 2013
By:
Geng Leng, Li Feng, Shao-Bo Li, Ping Yang, De-Zhong Dan
A novel method for analysing mercury in sediment is described. The technique involves microwave-assisted extraction followed by high performance liquid chromatography (HPLC) hyphenated to vapour generation atomic fluorescence spectrometry (VGAFS).
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April 1, 2013
By:
Michael W. Dong
The first instalment of the Perspectives in Modern HPLC column highlights some of the new HPLC systems, accessories and related technology introduced at Pittcon this year.
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March 1, 2013
There are many factors that influence the performance of a high performance liquid chromatography (HPLC) stationary phase, of which the chemical nature of the bonded phase ligand is important, but by no means all encompassing. Minor manufacturing parameters such as the method of electropolishing the internal surface of the column can also have an effect on the selectivity and efficiency produced by a particular column.
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February 18, 2013
By:
Incognito
Incognito found a 2D LC solution to a tricky separation problem but this technique did not meet the client's requirements. The magnanimous columnist admits to a rare defeat on this occasion but praises the thrill of seeking a solution to a perplexing method development problem. Could any readers suggest a more straightforward solution?
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August 1, 2012
By:
Laura Bush
In honor of LCGC's celebration of 30 years covering the latest developments in separation science, we asked a panel of experts (listed in the sidebar) to assess the current state of the art of liquid chromatography (LC) column technology, and to try to predict how the technology will develop in the future. This article is part of a special group of articles covering the state of the art in sample preparation, gas chromatography (GC) columns, GC instrumentation, LC columns, and LC instrumentation.
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April 19, 2012
By:
Kelly Ke Zhang, Midco Chi-Ying Tsang
Automated column and mobile phase screening is the current trend in the pharmaceutical industry to develop chiral methods. This study presents comprehensive strategies for automated rapid chiral HPLC method development including normal-phase, reversed-phase and polar organic phase separation modes.
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April 1, 2012
By:
Karin Cabrera
Monolithic columns feature both high column efficiency and high column permeability. This article discusses their preparation, applications, and future prospects.
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April 1, 2012
By:
Einar Pontén
Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized separations are described.
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