WEBCASTS


Tuesday, May 8, 2012
11:00 AM EDT
The Chemistry of Solid Phase Extraction

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This tutorial webcast will cover the basics of using solid-phase extraction sorbents, including explanations of how reversed-phase, normal phase, ion exchange, and mixed mode sorbents work, and how to use them. The terminology used will be easy to follow. Some discussion of applications and troubleshooting will be included.

Sponsored by: UCT


Wednesday, May 9, 2012
11:00 AM EDT
Increased Throughput for Trace Impurities and Residual Solvent Determination with Unattended Liquid/Headspace

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In this webinar, we will present the use of an innovative robotic autosampling platform which possesses the ability to automatically switch from liquid to headspace mode and use different syringe volumes in the same run or sequence onto a single GC. Test results of residual solvents quantification, where sample and standard preparation steps according to the USP method <467> procedures are carried out by the autosampler prior to the headspace analysis, will be shown. The lack of carryover achieved by the use of a headspace gas-tight syringe that is heated and flushed between injections will also be discussed.

Sponsored by:Thermo Fisher Scientific


Wednesday, May 16, 2012
11:00 AM EDT
A New Class of Photoresponsive Polymers that Undergo Controlled and Complete Polymer Chain Scission Upon Irradiation at 300nm

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Stimuli responsive materials are of interest in a number of applications. Of these, photoresponsive materials have a unique advantage since their response can be temporally controlled. We have developed a series of photodegradable phenacyl based polycarbonates. Hydroxyphenacyl based phototriggers, developed by Givens and coworkers, have been demonstrated to be a very efficient photoactivated protecting group for the release of esters, phosphates and thiols. We have incorporated this photoactive unit in the polymer backbone and irradiation of this polymer at 300 nm leads to polymer chain scission that results in substantial decrease in molecular weight. These polymers undergo photoinitiated degradation in both solution and the solid state. Well defined 2D patterns can be formed from films of these polymers by the use of simple masks. These polymers also have the potential for controlled release applications. As a model, the release of dyes such as rhodamine 6G or therapeutics such as indomethacin indicate controlled release upon irradiation of polymer films encapsulated with these molecules.

Sponsored by:TOSOH BIOSCIENCE


Thursday, May 17, 2012
11:00 AM EDT
High-Pressure IC for Increased Resolution in Beverage Analysis

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Carbonate beverages and fruit juices are complex matrixes containing organic acids, inorganic anions, and inorganic cations present as additives, as part of their natural sources, or as indicators of spoilage from biological growth. The determinations of these analytes are important to the beverage industry to verify content needed for labeling and formulations, to assess product freshness, protect health-sensitive consumers, or identify adulteration of a higher cost with a lower cost product. As such, ion chromatography is analytical method of choice to determine both inorganic anions and organic acids in a single run. Now, we introduce high pressure capabilities to capillary ion chromatography for our most flexible and advanced dual channel IC system, the Thermo Scientific Dionex ICS-5000 HPIC (high Pressure Ion Chromatograph) IC System, and to our new single channel ICS-4000 HPIC Integrated System for dedicated analysis. The high pressure capabilities, which allow continuous system pressures up to 5000 psi, bring the advantages of 2x faster runs and reduced cycle time to the capillary advantages of "IC on Demand" with only 5 L of waste generated a year. These high pressure capabilities also make it possible to use the new smaller 4 µm particle Thermo Scientific Dionex IonPac columns for very high resolutions and separations needed in complex sample matrixes. Here we discuss the advantages of high pressure capillary IC to provide highly resolved peak separations and determine important analytes in beverages.

Sponsored by:Thermo Fisher Scientific


Wednesday, May 23, 2012
2:00 PM EDT
EDITORS' SERIES: Optimizing GC Methods for Speed: More than Just Columns

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Most discussions of "fast GC" focus on developments related to columns: shorter, smaller inside diameters and thinner films. While the column is the "heart" of the method, the other components and steps in the method also have strong effects on analysis speed and time. In this web seminar, a brief overview of column-related strategies for improving GC method speed will be presented, followed by a discussion of implications on the other components of the method. Faster separations on the column impact all facets of the overall method: sampling, sample preparation, injection, detection and data analysis. Thinking about all of these areas enables chromatographers to more carefully consider how changes to the separation itself to increase speed should be approached. It is possible that the most important gains in the speed of the overall method may not come from the separation itself.

Sponsored by: SHIMADZU


Wednesday, May 23, 2012
9:00 AM EDT
PRIME: Next Generation Proteomics

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Proteomics analyses are among the most challenging analyses that can be addressed with mass spectrometry. The proteome dynamics in time, space and concentration as well as its variability because of modifications and mutations require novel and complementary approaches to generate useful, reliable and complete information. There is no "one size fits all" in unraveling the Proteome. PRIME delivers a multidimensional toolbox to unlock that complexity. Based on Bruker's ESI and MALDI mass spectrometer's class-leading performances and versatility, combined with cutting-edge bioinformatics solutions, PRIME has the potential to deliver answers where other solutions are just providing data. From bottom-up to top-down approaches, we will give an overview of the solution's capabilities in today's most challenging proteomics areas, such as identification, quantification and characterization from highly complex samples in proteomics and glycoproteomics, or tissue-based biomarker identification.

Sponsored by: Bruker Daltonics


Tuesday, June 5, 2012
10:00 AM EDT
Pushing the Limits in LC/MS-MS Sensitivity: Advanced Technology to Produce, Focus and Detect More Ions

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The modern LC/MS/MS laboratory continues to evolve year after year, seeking new ways to improve sensitivity, accelerate throughput and enhance data quality. This webinar will focus on revolutionary new LC/MS/MS technology that pushes the limits in sensitivity for challenging assays through advances in ionization efficiencies, ion transmission techniques, and next-generation detection systems.

Sponsored by: AB Sciex


Thursday, June 7, 2012
11:00 AM EDT
Using Automated Software for Improved Results in GC-Triple Quadrupole Pesticide Analysis

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In recent years, GC-triple quadrupole mass spectrometry has increased in popularity due to its ability to offer lower detection limits in complex matrices, simplified sample prep requirements, and faster analysis times. Of course, new instrument technology presents the need for the acquiring of new skills to harness the advantages offered by its adoption into current workflows.

New challenges that arise when using GC-triple quadrupole technology for complex analysis, such as pesticides in food, include the determination of the selected reaction monitoring (SRM) transitions that make up a typical MS/MS method and the maintenance of the acquisition windows when a large number of compounds are being monitored.

In this webinar, a strategy for addressing both of these challenges is discussed in the context of new software designed to automate common method development and method maintenance tasks. Also, in addition to making the triple quadrupole easier to use, this strategy can increase sensitivity of the analysis, which will be demonstrated using a complex SRM pesticide method as an example.

Sponsored by: Thermo Fisher Scientific

Tuesday, June 26, 2012
11:00 AM EDT
EDITORS' SERIES: A Roadmap for Rapid HPLC Method Development: A Practical Three-Pronged Approach

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HPLC method development is a challenging task, requiring the ability to optimize a large number of dependent and independent variables for complex samples. The traditional method development approach, while well accepted, is difficult to implement in practice, particularly for those with little experience. In this seminar, we describe the concept of a practical, three -pronged approach for rapid method development.

The template consists of three prongs or types of methods:
1. Fast LC sub-2-minute methods for potency assays or simple mixtures.
2. Generic broad gradient or in-process control (IPC) methods for simple molecules or samples.
3. Multi-segment gradient methods for stability-indicating assays or ICH-compliant impurity methods for complex molecules or samples.

This template approach is highly applicable to conventional HPLC or ultra-high-pressure LC and to all sample types. It is particularly attractive for rapid HPLC method development for new drug candidates in early pharmaceutical development. A case study of a complex molecule with multiple chiral centers will be used to illustrate the utility of this approach.

Sponsored by: SHIMADZU