Application Notes: LC/HPLC

Sep 09, 2017
Sponsored Content - EU
In this technical note, we report the chiral separation of racemic Naproxen using the immobilized polysaccharide-based chiral stationary phase Lux i-Amylose-1. Under optimal conditions, the racemic Naproxen was efficiently separated with a resolution factor of 2.33 and a separation factor (alpha) of 1.16. This method can be used for the enantiomeric separation and purity assessment of S-Naproxen, the active pharmaceutical ingredient (API).
Aug 31, 2017
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The Agilent 1290 Infinity II 2D-LC solution with an Agilent 6530 Accurate Mass Quadrupole Time-of-Flight LC/MS was used to determine critical differences between an infliximab originator and candidate biosimilar.
Aug 31, 2017
Sponsored Content - EU
The Agilent 1290 Infinity II 2D-LC solution with an Agilent 6530 Accurate Mass Quadrupole Time-of-Flight LC–MS was used to determine critical differences between an infliximab originator and candidate biosimilar.
Aug 22, 2017
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Different preparations of chondroitin sulfate have different molecular properties, which are related in turn to therapeutic effectiveness. Such properties can be studied—and quantified—by light scattering analysis, as described in this note.
Aug 22, 2017
Sponsored Content - EU
Different preparations of chondroitin sulfate have different molecular properties, which are related in turn to therapeutic effectiveness. Such properties can be studied—and quantified—by light scattering analysis, as described in this note.
Aug 21, 2017
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Typically, silica-based size-exclusion columns require high concentrations of non-volatile salts to facilitate timely elution, and require dedicated columns for GPC or GFC applications. Addressing these issues, our new size exclusion chromatography silica-based column utilizes volatile salts making it compatible with MSn, CAD or ELSD; provides excellent results in aqueous and/or organic mobile phases; and offers the mechanical stability of silica-based columns.
Aug 21, 2017
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Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance.
Aug 21, 2017
Sponsored Content - EU
Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance.
Aug 17, 2017
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Polar compounds, including those with biological interest, can be very hard to analyze by reversed-phase or HILIC HPLC or LC–MS. This application note shows how some sugars, sugar alcohols, and amino acids are readily and precisely retained by ANP HPLC using Cogent silica hydride columns.
Aug 17, 2017
Sponsored Content - NA
This application note shows a one-step analysis of a 20-mer DNA-based oligonucleotide, using a TSKgel SuperQ-5PW column. The effect of pH, temperature, and sample load on the selectivity and resolution, particularly in reference to the separation of N-1 and N+1 peaks from the main peak, are discussed. The aligned selectivity of this anion exchange column with its process scale counterparts makes the TSKgel SuperQ-5PW column extremely useful for the separation of oligonucleotides.
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