Application Notes: LC/HPLC

Jul 11, 2017
Sponsored Content - NA
This application note shows the seamless transfer of a method for the analysis of beta-blockers from an Agilent 1260 Infinity LC to an Agilent 1260 Infinity II LC.
Jul 10, 2017
Sponsored Content - EU
Direct analysis of 14 amino acids was performed with a straightforward HILIC–ESI-MS method. The column used was an iHILIC-Fusion(+) column packed with charge modulated hydroxyethyl amide silica. Mixed mode interactions such as hydrophilic partitioning, weak electrostatic interactions, and hydrogen bonding may be involved in the HILIC separations.
Jun 27, 2017
Sponsored Content - NA
Ligand binding assays (LBAs) used for large molecule drug development usually necessitate conjugation of a drug to one or more different tags. To help ensure that LBAs utilizing conjugated drug reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically prior to being incorporated into the LBA.
Jun 27, 2017
Sponsored Content - EU
Ligand binding assays (LBAs) used for large molecule drug development usually necessitate conjugation of a drug to one or more different tags. To help ensure that LBAs utilizing conjugated drug reagents perform consistently over time and provide high quality data, each lot of conjugated material should be characterized functionally and biophysically prior to being incorporated into the LBA.
Jun 27, 2017
Sponsored Content - NA
By Advion
The expression CMS is a novel compact mass spectrometer with faster scan speed, on-line polarity switching, and in-source CID. This TLC–FIA–MS workflow can generate the required information for compound identification and structural information of analytes from natural products and medicinal compounds derived from plant material.
Jun 26, 2017
Sponsored Content - NA
Shodex PROTEIN LW-803 column has a durable silica-based packing material ideal for antibody SEC analysis. This column has an adopted pore size suitable for analyzing proteins with a molecular weight of several hundreds of thousands, corresponding to the USP L20, L33, and L59.
Jun 22, 2017
Sponsored Content - NA
The advantage of UV spectroscopy as an analytical method to detect protein aggregation is that it is non-destructive and uses low sample volumes, minimal sample preparation requirement, and easy sample analysis. A quick QC method to indicate the presence of aggregation uses an Agilent Cary 60 UV-vis spectrophotometer to identify aggregation of mAb solutions resulting from different stress conditions.
Jun 21, 2017
Sponsored Content - NA
Accurate mass determination of a bispecific T cell engager (BiTE®) consisting of two single-chain variable fragments (scFvs) recombinantly linked by a nonimmunogenic five-amino-acid chain was analyzed by SEC–MS using a TSKgel® UP-SW3000, 2-µm column under non-denaturing conditions. No signs of particle shedding or sample carryover, which may interfere with MS signal response, were noted in the analysis.
Jun 20, 2017
Sponsored Content - NA
Intact and reduced IgGs have been successfully retained and separated using a non-porous column. Potential applications of Presto columns for a single-injection separation of free drug, antibody, and antibody-drug complexes (ADCs) are also discussed.
Jun 19, 2017
Sponsored Content - EU
Neonicotinoids are a relatively new class of insecticide that were introduced as an alternative to organophosphate, carbamate, and pyrethroid insecticides. Their novel mode of action works by irreversibly binding to nicotinic acetylcholine receptors, resulting in paralysis and death of insects. This application note outlines a simple, fast, and cost-effective method for the determination of seven neonicotinoid pesticides in honey.
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