LCGC Blog

Nov 07, 2017
I hear the words “struggling for sensitivity” so often when speaking to folks using LC–MS for bioanalysis, environmental analysis, metabolomics, proteomics, and a host of other applications where target analytes are present at low concentrations in complex matrices. We spend fortunes on MS/MS instruments to increase specificity of detection in order to improve sensitivity. Some of us go to great lengths to optimize sample extraction and HPLC conditions in order to minimize matrix suppression effects and improve specificity and hence sensitivity.
Oct 30, 2017
The presence of capable students and researchers, each with a technical niche to offer, prepares us for future opportunities. On top of that structure, we also aim to maintain this mindset for our instrument selection before beginning projects. The installment of core labs at UT Arlington (www.uta.edu/sirt), making a wide range of instruments available to all research groups, has allowed our students to brainstorm about which instrument is the most appropriate for a specific analysis rather than how to make a measurement work with a given instrument.
Oct 05, 2017
Data validity and a thorough understanding of the results that we produce should be of great interest to us all. Here's why.
Sep 27, 2017
Internal standards (IS) are commonly incorporated into quantitative methods to increase accuracy and precision. An IS is a compound that is different than the analyte of interest, has similar physicochemical properties to the analyte, and is added to samples, calibration standards, and quality control samples in a known quantity. It should not be present in the sample, it should be available in high purity, and it should be easily differentiable from the analyte of interest.
Sep 12, 2017
With the increasing proliferation of UHPLC, with its very much reduced extra column volumes, is the concept of dwell volume still relevant? Indeed, in your world, was it ever relevant at all?
Sep 12, 2017
The Column
The ability to rapidly screen stationary phases through column-switching capabilities provides significantly greater efficiency in method development than was previously possible. The approach does require some additional hardware and software. And, while such capabilities may limit the ability to expand one’s literary knowledge during excessive months in the laboratory developing separation methods, real progress to key decision points for method optimization can be realized instead.
Aug 29, 2017
As the fall semester starts, I begin my 13th year as a chemistry professor. Let me get right to the point—if you do not love this job, you will hate it!
Aug 07, 2017
The ability to rapidly screen stationary phases through column-switching capabilities provides significantly greater efficiency in method development than was previously possible. The approach does require some additional hardware and software. And, while such capabilities may limit the ability to expand one’s literary knowledge during excessive months in the laboratory developing separation methods, real progress to key decision points for method optimization can be realized instead.
Jul 26, 2017
Until recently, I hadn’t heard of ternary or quaternary gradients being used for many years. They have gained a reputation for being somewhat difficult to reproduce—less robust, if you like.
Jul 10, 2017
The LCGC Blog
Hydrophilic Interaction Chromatography can be a very useful tool in our analytical armory, however there are some practical peculiarities to this chromatographic mode which need to be understood in order to ensure success. 
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