LCGC Blog

Dec 09, 2015
E-Separation Solutions
It is much more efficient to screen column chemistries to achieve optimum separations for a set of target analytes, than it is to play with different mobile-phase conditions on a single column. Kevin Schug explains more.
Nov 22, 2015
The Column
LCGC Blogger Tony Taylor offers some simple visual methods to assess column performance.
Nov 17, 2015
They say a picture paints a thousand words… This month I’ve taken inspiration from a recent webcast, presented at www.chromacademy.com, in which I presented real data from our work that represents some ‘classic’ GC problems.
Nov 10, 2015
Column overload is a very commonly encountered issue in GC for beginners. Changes in peak symmetry, generally observed as peak fronting, can be subtle in the sharp peaks generated by GC, but the result can be significant shifts in retention times, loss of resolution, and error in peak integration.
Nov 06, 2015
The Column
Kevin Schug discusses why we need a comprehensive study of unconventional oil and natural gas (UOG) extraction processes.
Oct 12, 2015
A client asked me recently for a quick but "as optimized as possible" separation for some monoclonal antibody (MAb) characterization (digest) samples using LC-MS. The samples had been prepared and were awaiting analysis—typical forward planning! Actually his internal analytical department had changed their priority and he was left hanging.
Oct 07, 2015
If you want to improve analytical methods to make them better, the sample preparation step is probably one of the best steps to target.
Sep 14, 2015
Mobile-phase preparation for LC–MS requires careful consideration to ensure the correct pH values and concentrations are obtained. This blog installment details the difference between using volume percent and weight percent to to make up 0.1% solutions of trifluoroacetic acid and the effect it has on the pH.
Sep 09, 2015
There is a shortcoming in our current educational system. There is too much rote learning, and not enough time given to let science-minded students explore a topic. Overall, when students ask their own questions (not ones given to them by instructors), they become more invested in finding the answers.
Aug 18, 2015
Tony Taylor provides an exercise to highlight the ease with which instrument error in our data can be estimated when using methods involving linear regression. He also discusses the importance, under certain circumstances, of our clients (internal or external) and us being aware of the true precision of our data.
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