Column: Focus on Biopharmaceutical Analysis

Jan 01, 2015
LCGC North America
Most analytical approaches used for the determination of low-molecular-weight noncovalent aggregates in protein pharmaceuticals suffer from important limitations. This first part of this two-part column series discusses those limitations.
Dec 01, 2014
LCGC North America
In this installment, we discuss fluorescence-detection size exclusion chromatography (FSEC) technology and its implications in protein characterization, specifically the development and characterization of biologics.
Sep 01, 2014
LCGC North America
A discussion of the role of the gradient in separation by reversed-phase and ion-exchange HPLC, with data from two products to illustrate the key points
Jan 01, 2014
LCGC North America
The modern usage and specific applications of HILIC for two other major classes of analytes — glycopeptides and glycoproteins — are discussed.
Dec 01, 2013
LCGC North America
Answers to common questions about HILIC, and an overview of its application to biopharmaceutical analysis.
Nov 01, 2013
LCGC North America
The use of a monolithic column reduces analysis time in an on-line biopharmaceutical HPLC analysis of granulocyte colony stimulating factor.
Jan 01, 2013
LCGC North America
How to use these tools to develop an efficient and robust process
Dec 01, 2012
LCGC North America
Detailed examples of light-scattering techniques are presented as well as how their performance compares to MS and other methods.
May 01, 2012
LCGC North America
LC–MS and CE–MS are the go-to techniques for characterizing biologics, but often help is needed from a range of other methods.
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