Column: Column Watch

Apr 01, 2018
LCGC North America
Our annual review of new liquid chromatography columns and accessories, including columns for reversed-phase, HILIC, chiral, SEC, GPC, ion-exchange, and SFC separations of small and large molecules.
Feb 01, 2018
LCGC North America
Despite the theoretical promise of reduced plate heights (h) < 1, most modern UHPLC columns can only deliver plate heights in the range from 1.4 to 2.5. However, improved packing procedures, a better understanding of the colloidal properties of particle suspensions, and the study of the rheological behavior of packed beds and the final bed structure should lead us to practical solutions that can double the column efficiencies.
Dec 01, 2017
LCGC North America
A number of approaches for peak shape measurement are available in modern chromatography software. We discuss the advantages and drawbacks of those approaches, and present a new concept, “total peak shape analysis.”
Oct 01, 2017
LCGC North America
To resolve and quantitate monoclonal antibodies and antibody–drug conjugates, analytical scientists need various chromatographic modes, such as size-exclusion, ion-exchange, hydrophobic interaction, and hydrophilic interaction chromatography. Here, we provide advice for making the most of each mode.
Sep 01, 2017
LCGC North America
Topics that stood out at HPLC 2017 include advances in large-molecule separations, multidimensional chromatography, HILIC, and chiral separations.
Jul 01, 2017
LCGC North America
A model set of analytes and selected applications are used to demonstrate the effects that buffers can have on the selectivity of a separation and the sensitivity of a reversed-phase analysis when using MS detection.
Jun 01, 2017
LCGC North America
In biphenyl phases, the surface chemistry often makes it possible to separate compounds not well resolved by C18 or phenyl phases.
Apr 01, 2017
LCGC North America
Our annual review of new liquid chromatography (LC) columns and accessories, including an analysis of the overall trends.
Feb 01, 2017
LCGC North America
This interactive classification map is a powerful tool for gaining useful knowledge about the properties of bonded LC stationary phases.
Dec 01, 2016
LCGC North America
The study shows results that promise to improve HILIC method development practices and provide insight into retention mechanisms across mechanistically different polar stationary phases.
lorem ipsum