Antiepileptic drugs (AEDs) are prescribed to suppress seizures in epilepsy patients. The ability to therapeutically monitor these drugs in patients is necessary for maintaining optimal medical care and managing any adverse effects of the drug. In this application note ISOLUTE SLE + is demonstrated as an effective way to extract AEDs from serum and urine. The method was developed using a 96-well plate format to facilitate a high throughput workflow model.
Format: ISOLUTE® SLE+ 400 μL 400 Supported Liquid Extraction plate, part number 820-0400-PO1Sample pre-treatment: Pipette serum/urine (blank, calibrator, and patient [100 μL]) into a container. Add 50% aqueous formic acid (100 μL). Add up to 100 μL of internal standard.
Sample processing: Load up to 300 μL of pre-treated serum sample onto the ISOLUTE SLE+ 96-well plate. Apply a pulse of positive pressure or vacuum and allow samples to sit for 5 min.
Analyte elution: Elute analytes with methyl tert-butyl ether containing 1% (v/v) trifluoroacetic acid (conc) solution (2 × 700 μL). Allow sample to flow through by gravity and collect eluent. Apply positive pressure or vacuum as needed to facilitate a constant flow of approximately 1 mL/min (10–12 drops).
Instrument: Agilent 1200 Liquid Handling System (Agilent Technologies, Berkshire, UK)
Column: Phenomenex Gemini C18, 150 mm × 4.6 mm (5 μm)
Mobile phase: Solvent A: 5 mM ammonium formate with 0.01% (v/v) formic acid; Solvent B: methanol:acetonitrile (50:50 m v/v)
Mass Spectrometry Conditions
Instrument: Applied Biosystems/MDS Sciex 4000 Q-Trap triple quadrupole mass spectrometer (Applied Biosystems, Foster City, California, USA) equipped with a Turbo Ionspray® interface for mass analysis.
Ion source temperature: 700 °C
Results and Discussion