One of the world's premier conferences and expositions on laboratory science, the Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy (Pittcon) is a showcase for the latest advances in laboratory instrumentation and technology, attracting all of the major high performance liquid chromatography (HPLC) vendors in addition to more than 900 exhibiting companies from around the world. Pittcon is a great place for vendors to introduce, and scientists to see, touch, evaluate, compare, and hear all about the latest in HPLC technology. This installment will highlight some of the new HPLC and related technology showcased both before and at this year's conference.
This year, the Pittsburgh Conference on Analytical Chemistry & Applied Spectroscopy (Pittcon) was held on March 11–15 at the Orange County Convention Center in Orlando, Florida. The conference, which annually attracts more than 15,000 attendees from industry, academia, and government from 90 countries worldwide, is sponsored by the Spectroscopy Society of Pittsburgh and the Society for Analytical Chemists of Pittsburgh. Pittcon 2012 had more than 900 exhibitors registered to appear in more than 1800 booths. Upwards of 2000 technical presentations and nearly 100 short courses also were given. The conference provides a great opportunity for vendors to expose new high performance liquid chromatography (HPLC) products to both new and existing customers, and with this fact in mind, many HPLC vendors have coordinated their new product development cycle around Pittcon for years, introducing new technology at the conference for the first time. Although the proliferation of smaller specialized conferences has in recent years altered this approach, for example, many new mass spectrometry (MS) product introductions are now made at the annual Conference on Mass Spectrometry and Allied Topics (ASMS), Pittcon still remains a must-go-to meeting to see the latest and greatest in HPLC and related technology. However, this year marked the first time that at least one HPLC vendor decided to go completely virtual, and instead of attending Pittcon, chose to offer a series of local seminars and on-line web-based e-conferences instead. For the attendees, the conference provides a venue to evaluate the latest instrumentation, compare vendors, participate in product demonstrations, and speak with technical staff to resolve problems or investigate potential applications. It will be interesting to see how things play out in the virtual world.
Supercritical fluid chromatography (SFC) has been commercially available since about 1982, but more recently it has gained in popularity because of advances in technology and applications to several difficult separation challenges (for example, the separation of chiral compounds). SFC is a form of normal-phase chromatography that is used for the analysis and purification of low- to moderate-molecular-weight, thermally labile molecules. The theory and principles of SFC are similar to those of HPLC; however, SFC can be differentiated from other chromatographic techniques (gas chromatography [GC] and HPLC) by the use of a supercritical fluid as the mobile phase and, therefore, the entire chromatographic flow path must be pressurized. SFC has several advantages compared to GC and HPLC methods, including rapid separations without the use of organic solvents, reduced use of organic additive chemicals, and the use of carbon dioxide collected as a by-product of other chemical reactions (or collected directly from the atmosphere), thereby contributing no new chemicals to the environment and promoting "green" chemistry. In addition, SFC separations can be done faster than HPLC separations because the diffusion of solutes in supercritical fluids is about 10 times greater than that in liquids (and about three times less than in gases). The lower diffusion results in a decrease in resistance to mass transfer in the column and allows for fast, high-resolution separations. Compared to GC, capillary SFC can provide high-resolution chromatography at much lower temperatures. This allows for the fast analysis of thermolabile compounds. Many vendors have recognized these advantages and have started to offer SFC instruments on existing HPLC and ultrahigh-pressure liquid chromatography (UHPLC) platforms.
The new Aurora SFC Fusion A5 Evolution system is built on the original Aurora SFC Fusion A5 instrument and features a combination of optimized pumping technology with an ultralow-noise back-pressure regulator. The upgrade kit allows full conversion of an Agilent 1100 or 1200 series HPLC system to a full analytical SFC system that moves SFC smoothly into routine laboratory analyses with enhanced features for full qualification in a good laboratory practice (GLP) or good manufacturing practice (GMP) environment. With cost containment being the focus of many companies, conversion can reduce the overall cost of entry. Four orders of magnitude of dynamic range provide accurate quantitation of 0.01%-level impurities, high precision, HPLC-like sensitivity, and UHPLC speed using UHPLC columns. The system is fully compatible with the manufacturer's chromatography data system software.
At past shows, Waters has showcased its SFC system, the Waters Acquity UPSFC system that is built on the Waters UPLC sub-2-µm particle chemistry platform. The UltraPerformance SFC (UPSFC) system is designed to incorporate all of the advantages of SFC, in addition to superior separation, resolution, and speed afforded by the sub-2-µm particle chemistry platform. This year, Waters introduced the UltraPerformance Convergence Chromatography Acquity UPC2 system, which gives scientists the ability to precisely vary mobile-phase strength, pressure, and temperature. With the ability to fine-tune the resolving power and selectivity of the system, scientists can exercise better control over the retention of analytes for separating, detecting, and quantifying structural analogs, isomers, and enantiomeric and diasteriomeric mixtures — all compounds that are often difficult to separate by any other means. Its key features include