LCGC Asia Pacific June 2016

June 2016 | Volume 19, Issue 2
Cover Story
Nontargeted metabolite profiling by ultrahigh-pressure liquid chromatography–mass spectrometry (UHPLC–MS) is a powerful technique to investigate the influence of genetic and environmental factors on metabolic phenotypes in plants. The approach offers an unbiased and in-depth analysis that can reveal molecular markers of desirable phenotypic traits that can be complementary to genetic markers in plant breeding efforts. Here, the power of nontargeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley that are predictive of desirable malting quality for brewing applications.
Appropriate analytical methods are required to evaluate the presence, metabolism, degradation, and removal of specific compounds in complex mixtures. There is an increasing demand to analyze samples with a wide range of polarities in a variety of applications, including environmental analysis, biomarker discovery, and proteomics. Multiple analyses on complementary columns are often needed to cover the separation of all compounds with a large difference in polarity. This article describes a generic method involving an ultrahigh‑pressure liquid chromatography (UHPLC) system equipped with two external switching valves to connect hydrophilic interaction liquid chromatography (HILIC) and reversed-phase LC columns in series for the sequential analysis of polar and apolar compounds.
Understanding how liquid chromatography pumps operate can help streamline solving pump problems.
John Hinshaw presents his annual review of new developments in the field of gas chromatography (GC) seen at Pittcon and other venues in the past year.
By LCGC Editors
The 27th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2016) will be held in Guangzhou, China, from 13–16 November 2016.
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