LCGC Asia Pacific November 2015

November 2015 | Volume 18, Issue 4
Cover Story
This article presents a method for comparing the levels of baseline interference arising from common laboratory mobile phase contamination sources and assesses different approaches for removing dissolved contaminants to generate interference-free chromatogram baselines. The authors demonstrate that recirculating mobile phase through a semi-preparative scale column using a reagent delivery pump has advantages over previously published mobile phase decontamination methods.
LC Troubleshooting
Are your solvent costs too high? Here are some ideas on how to decrease the amount of mobile phase you use.
GC Connections
In gas chromatography (GC) split injection systems, as much as 99% or more of the injected sample never enters the column: It is released downstream of the inlet where it can encounter and possibly perturb precision gas control devices. The split-vent trap acts to prevent or at least moderate such effects. This month’s instalment addresses the operation and maintenance of split-vent traps.
Column Watch
Hydrophilic-interaction chromatography (HILIC) is a complex system involving partition, polar, and ion‑exchange interactions. Method development can be greatly facilitated by understanding the interactions that the different stationary phases provide and applying that knowledge to the separation task at hand. Through an understanding of the main differences of the set of analytes to be separated, a column or set of columns can be judiciously chosen to screen for effective retention and selectivity.
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