The LCGC Blog

Sep 13, 2016
I’ve often written about the “lazy” chromatography which has swept through our industry, whereby 0.1% (w/w or w/v!) TFA or formic acid is used to “buffer” the eluent system well away from the pKa of analyte molecules, leaving most acidic analytes in the ion suppressed form and most basic analytes in the ionized form. This approach avoids having issues with retention time drift and effectively eliminates pH as a variable used to control retention or selectivity for separations involving ionogenic analytes.
Sep 07, 2016
The LCGC Blog
In a recent review of blood alcohol casework performed by a forensics laboratory associated with a major metropolitan police force, I was again disheartened to find major deficiencies in method validation protocols. In this case, the analysts failed to check whether aqueous solutions for calibration and quality control were reliable surrogates for real blood samples.
Aug 16, 2016
I’ve done that thing where I’ve stated a very interesting title—I hope I can deliver something which lives up to it. I dislike it when people “overstate” their talk or poster titles at conferences to draw me in and then don’t deliver against the promise—I’ll let you judge how we go here.
Aug 09, 2016
Top-down protein quantitation, especially using triple-quadrupole MS, but even in general, has hardly been pursued. To help fill this gap, we recently reported a systematic investigation of intact-protein quantitation using multiple reaction monitoring (MRM) on a triple-quadrupole MS system, and we believe that this approach can be a promising alternative route to consider going forward.
Jul 15, 2016
The LCGC Blog
The title of this piece may have put you off reading it – in which case you won’t be insulted when I say that even the most experienced gas chromatographers often fail to install columns in the best way possible.
Jul 12, 2016
The LCGC Blog
Matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is actually one of the most versatile tools for fingerprinting mixtures.
Jun 14, 2016
I believe that most analytical chemists do not appreciate what the world of chemometrics and experimental design statistics can add to their work.
Jun 07, 2016
Not every method is as we would hope it would be. Some methods come to us in imperfect form and we have to live with them, while others are difficult separations and, by necessity, need to be developed using close control of several variables such as eluent pH, eluotropic strength, ionic strength, and so on.
May 17, 2016
In 2009 I wrote an article on the emerging field “Bio Chromatography,” which for a small molecule analyst such as myself, perfectly described the situation. I realize that the separation of biomolecules had been happening for many years, but the expansion and development of protein based therapeutics from that point onwards has seen an avalanche of developments in instrumentation, sample preparation, and column technologies the like of which I have not seen in my 30 year career.
May 11, 2016
Compared to the magnitude of unconventional oil and gas development activity that has been performed by industry, the amount of research to assess its potential environmental impact has been miniscule. Can the process have a deleterious impact if not well managed? Can it be performed in a responsible manner?
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