The LCGC Blog

Nov 17, 2015
They say a picture paints a thousand words… This month I’ve taken inspiration from a recent webcast, presented at, in which I presented real data from our work that represents some ‘classic’ GC problems.
Nov 10, 2015
Column overload is a very commonly encountered issue in GC for beginners. Changes in peak symmetry, generally observed as peak fronting, can be subtle in the sharp peaks generated by GC, but the result can be significant shifts in retention times, loss of resolution, and error in peak integration.
Oct 12, 2015
A client asked me recently for a quick but "as optimized as possible" separation for some monoclonal antibody (MAb) characterization (digest) samples using LC-MS. The samples had been prepared and were awaiting analysis—typical forward planning! Actually his internal analytical department had changed their priority and he was left hanging.
Oct 07, 2015
If you want to improve analytical methods to make them better, the sample preparation step is probably one of the best steps to target.
Sep 14, 2015
Mobile-phase preparation for LC–MS requires careful consideration to ensure the correct pH values and concentrations are obtained. This blog installment details the difference between using volume percent and weight percent to to make up 0.1% solutions of trifluoroacetic acid and the effect it has on the pH.
Sep 09, 2015
There is a shortcoming in our current educational system. There is too much rote learning, and not enough time given to let science-minded students explore a topic. Overall, when students ask their own questions (not ones given to them by instructors), they become more invested in finding the answers.
Aug 18, 2015
Tony Taylor provides an exercise to highlight the ease with which instrument error in our data can be estimated when using methods involving linear regression. He also discusses the importance, under certain circumstances, of our clients (internal or external) and us being aware of the true precision of our data.
Aug 11, 2015
We recently published a study of water quality from 550 water wells in the Barnett Shale of North Texas. Using a suite of analytical techniques, we were able to find new evidence of the potential impact of unconventional natural gas extraction on environmental quality.
Jul 14, 2015
Using ESI-MS to perform quantitative binding analyses and determine association constants depends on the ability of the ionization process to preserve the system equilibrium.
Jul 07, 2015
E-Separation Solutions
If you use SPE in your work, then most likely it’s very important to the success of your applications, and its proper implementation will be key to the performance of your analyses. In an effort to help you develop a better understanding of the technique, LCGC blogger Tony Taylor offers eight steps to solid-phase extraction success.
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