LCGC Europe February 2017

February 2017 | Volume 30, Issue 2
Cover Story
Electrophoretic concentration (EC) is an electric field-driven and environmentally friendly off-line sample preparation for charged analytes. EC was demonstrated for the enrichment of either six anionic pollutants or five cationic drugs from purified, drinking, river, or waste- water samples. EC provided analyte enrichment in 15–50 min with concentration factors of 30–249 and 12–243 for the negatively and positively charged analytes, respectively. A modification of the EC device enabled simultaneous EC and separation (SECS) of six cationic and anionic herbicides with concentration factors of 18–337 in 30 min. The potential of SECS has also been evaluated for the determination of high mobility ions in urine and the results obtained have been compared to common acetonitrile treatment of urine. SECS provided an enrichment of high mobility ions and revealed more peaks compared to the acetonitrile treatment.
This article describes a space-saving, quick, and inexpensive sample preparation technique followed by a high performance liquid chromatography (HPLC) method with a 100% water mobile phase and photodiode array (PDA) detection for quantifying acetamiprid and its N-desmethyl metabolite, IM-2-1, in cow’s milk. The analytes were extracted from the sample and deproteinized using a handheld ultrasonic homogenizer with 5% (w/v) trichloroacetic acid solution, purified using a centrifugal monolithic silica spin minicolumn, and quantified within 20 min per sample. The accuracy and precision are well within the international method acceptance criteria.
History of Chromatography
Peter Myers from the University of Liverpool (Liverpool, UK) spoke to David McCalley from the University of the West of England (Bristol, UK) about the past, present, and future of stationary phases, and his working life in academia and industry.
LC Troubleshooting
Delivering samples to the analytical column in “clean” mobile phase is important for robust methods and high-quality results.
Questions of Quality
Raw data is a term that is used in both good manufacturing practice (GMP) and good laboratory practice (GLP) laboratories but it can create misunderstanding. What exactly does raw data mean and what electronic records are within the scope of the term?
Column Watch
The carotenoid test allows one to build a simple classification map of stationary phases used in reversed‑phase liquid chromatography, on the basis of the shape recognition (plotted on the x axis), the polar surface activity (plotted on the y axis), and the phase hydrophobicity (related by the bubble size).
By LCGC Editors
The 8th International Symposium on Recent Advances in Food Analysis (RAFA 2017) will take place in Prague, Czech Republic, on 7–10 November 2017.
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