LCGC North America March 2003

March 2003 | Volume 21, Issue 3
LC Troubleshooting
John Dolan provides 10 tips for the proper care and use of LC columns.
Column Watch
By LCGC Editors
The first of two columns about new chromatography columns, accessories, and sample preparation products introduced at Pittcon 2003.
GC Connections
John Hinshaw examines the effects of system time constants and sampling rates on apparent peak shapes and areas.
Directions in Discovery
By LCGC Editors
Two solutions to the problem of obtaining quantitative information about protein expression are to couple two or more chromatographic modes to increase resolution and to use affinity selection techniques.
This article reviews several common applications of the critical-pair approach to column chromatography as it is applied under pharmaceutical guidelines and validation strategies.
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