LCGC North America July 2016

July 2016 | Volume 34, Issue 7
Column Watch
Microscale LC–MS use is now feasible on a much wider scale.
LC Troubleshooting
With good maintenance, an autosampler should run trouble-free.
GC Connections
Choosing the wrong septa can compromise method accuracy.
MS–The Practical Art
This direct-analysis MS method is more accurate than immunoassay screening and requires no special instrumentation.
Biotechnology Today
In this column, we introduce the basics of today’s approaches for doing intact protein dissociation with mass spectrometry (MS), or top-down sequencing (that is, rather than the more conventional peptide-based “bottom-up” sequencing where future improvements might occur, advantages and limitations of using top-down sequencing, possible applications, and why it has become such an important and pursued research area for many.
The Essentials
Tips for choosing the right GC columns and detectors.
Peer-Reviewed Article
How does an overcoated PDMS–DVB fiber compare to a nonovercoated one in a complex matrix? And how does the SPME method with the overcoated fiber compare to QuEChERS?
Application Note (Advertising Content)
This application note demonstrates the analysis of charge variants of Rituximab (both innovator and biosimilar molecules) using multiple heart-cutting two-dimensional liquid chromatography (2D-LC) with mass spectrometry (MS) detection.
lorem ipsum