LCGC North America December 2014

December 2014 | Volume 32, Issue 12
Peer-Reviewed Articles
This article reports on the physical phenomena that control column efficiency and on experimental protocols designed to accurately measure their contributions to band broadening of analytes during their passage from the injection to the detection device. The results of these protocols are analyzed, allowing for the accurate determination of the complete mass transfer mechanism in different separation modes and providing solutions and future directions to further improve the efficiency of liquid chromatography columns.
Sample Prep Perspectives
In this installment of "Sample Prep Perspectives," we cover some of the basic scientific principles behind solid-phase extraction (SPE) to allow the correct mode of extraction to be selected through an understanding of how analytes interact with and are separated by the sorbent.
LC Troubleshooting
Sometimes it may seem like you are spending most of your laboratory budget buying liquid chromatography (LC) columns. Why can't column manufacturers make a product that lasts longer? Actually, with reasonable care, the column should be a small proportion of the total LC analysis costs.
Biotechnology Today
In this installment, we discuss fluorescence-detection size exclusion chromatography (FSEC) technology and its implications in protein characterization, specifically the development and characterization of biologics.
The Essentials
For a robust method, each step must be carefully considered and optimized. The method specification must also provide sufficient detail to ensure reproducibility by different operators. How well do your methods hold up against our blueprint?
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