Characterization of Polyvinylcarbazole by SEC with MALS - - Chromatography Online
Characterization of Polyvinylcarbazole by SEC with MALS


The Application Notebook

Polyvinylcarbazole (PVK) is a photovoltaic polymer, which means that the bulk polymer — after being exposed to visible or ultraviolet light — becomes an electrical conductor. PVK is used in multilayer light-emitting devices to increase the transporting layer which improves the effi cicency of the device. It also finds uses in xerography and other electro-optical applications.

For the application of polymer materials containing PVK, the knowledege of its molecular weight and its molecular weight distribution (MWD) is of principal importance. Size-exclusion chromatography (SEC) was chosen for the analyses of the PVK samples. As PVK is a rather unusual polymer, with no calibration reference standards, the light-scattering detection was necessary to make possible the calibration of the separation system by the on-line detection of molecular weight.


Figure 1: Comparison of the calibration dependence constructed from the MALS-RI detection data for the PVK sample with RI signal showing the range of the calibration of the separation system.

Figure 2: Cumulative MWD constructed from the MALS-RI detection data for the PVK sample.
















For the analysis described in this application note, the DAWN-DSP multi angle light scattering (MALS) was connected to an SEC system working with tetrahydrofuran (THF) as the mobile phase. The analysis revealed that calibration was linear in the range of two orders of magnitude (Figure 1) even when the MWD is not terribly broad (M w /M n = 2.234, M w = 1.392 105). The cumulative MWD constructed from the lightscattering and differential refractive index (RI) data (Figure 2) makes it possible to find the fraction (between 0 and 1) of the material with molecular weight lower than the abscissa of the particular point on the cumulative MWD and subsequently modify further processing of the PVK samples intended for electro-optical applications.

Aknowledgement

This note was graciously submitted by Milos Netopilik, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic.

Milos Netopilik, Institute of Macromolecular Chemistry, Academy of Sciences or the Czech Republic, (on behalf of Wyatt Technology Corporation, Santa Barbara, California, USA).











Wyatt Technology Corporation
6300 Hollister Avenue, Santa Barbara, California 93117, USA
tel. +1 805 681 9009 fax +1 805 681 0123
E-mail:
Website: http://www.wyatt.com/

ADVERTISEMENT

blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:




 

LCGC COLUMNISTS 2014

Sample Prep Perspectives | Ronald E. Majors: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments.
LATEST: UV Detector Problems


Perspectives in Modern HPLC | Michael W. Dong: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Superficially Porous Particles: Perspectives, Practices, and Trends


MS — The Practical Art | Kate Yu: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting | John Dolan: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: Problems with Large-Molecule Separations


More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>


Source: The Application Notebook,
Click here