Characterization of Tobacco Extracts by Gas Chromatography High Resolution Time of Flight Mass Spectrometry (GC-HRT) - - Chromatography Online
Characterization of Tobacco Extracts by Gas Chromatography High Resolution Time of Flight Mass Spectrometry (GC-HRT)


The Application Notebook
pp. 14

High resolution time-of-flight mass spectrometry was used for comprehensive profiling of tobacco plant extracts.

The flavor and health risks of tobacco products are a direct result of their chemical composition. Alkaloids, organic acids, amino acids, saccharides, terpenes, and volatile aromatics are important contributors to tobacco leaf quality. It is well known that tobacco quality can vary significantly depending on where it is harvested. Comprehensive profiling is critical for understanding the chemical complexity responsible for aroma and flavor differentiation of tobacco leaves grown under different environmental conditions.

Experimental



Green leaf and cured tobacco extracts were analyzed using the Pegasus GC-HRT. The analysis workflow included EI-HRT and CI-HRT data acquisition to maximize the number of confidently identified metabolites. Instrument parameters are listed below:

Results and Discussion


Table I: Representative compounds in green leaf tobacco extract
Some of the major components in green leaf tobacco are terpenes, terpenoids, and sterols. An expanded analytical ion chromatogram (AIC) shows some of the plant sterols in the sample including campesterol, stigmasterol, sitosterol, and ?-Amyrin. The extracted ion chromatogram (XIC) illustrates the deconvolution power of LECO's ChromaTOF HRT software. The tocopherol and cholesterol MS spectral data is easily separated to provide high quality spectral data as shown by the excellent library match (LM = 880/1000) and mass accuracy value (-0.98 ppm) for cholesterol.


Figure 1: AIC (B) and XIC (A) of a green leaf tobacco extract. Peak true mass spectrum of cholesterol (C).
Tabular data for a representative set of additional compounds in the extract are listed in Table I. The quality of spectral data obtained using the GC-HRT is clearly evident from good library similarity matches (758–895/1000) and excellent mass accuracy values (Avg. = 0.95 ppm) which aided in library match confirmation for these analytes.

Conclusion

The Pegasus GC-HRT is an ideal tool for metabolomic profiling of plant extracts. Excellent mass accuracy values and high quality spectral data facilitated characterization of numerous classes of compounds in tobacco. For brevity, the CI-HRT data was not shown in this note, but will be included in a full application note at http://LECO.com/.




LECO Corporation
3000 Lakeview Avenue, St. Joseph, MI 49085
tel. (269) 985-5496, fax (269) 982-8977
Website: http://www.leco.com/

ADVERTISEMENT

blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:




 

LCGC COLUMNISTS 2014

Sample Prep Perspectives | Ronald E. Majors: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments.
LATEST: UV Detector Problems


Perspectives in Modern HPLC | Michael W. Dong: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Superficially Porous Particles: Perspectives, Practices, and Trends


MS — The Practical Art | Kate Yu: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting | John Dolan: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: Problems with Large-Molecule Separations


More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>


Source: The Application Notebook,
Click here