Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of
endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation,
Table II: Compounds
Steroids share a characteristic, polycyclic structure and have varying degrees of lipophilicity (log P). In this study, a
reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from
1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.
Figure 1: Separation of five steroids on a 4.6 × 150 mm PRP-C18.