Keeping Water Safe: Detecting Pharmaceutical and Personal Care Products in Water Using Liquid Chromatography–Mass Spectrometry - - Chromatography Online
Keeping Water Safe: Detecting Pharmaceutical and Personal Care Products in Water Using Liquid Chromatography–Mass Spectrometry


The Application Notebook
pp. 381382

The Problem with PPCPs

Pharmaceutical and personal care products (PPCPs) are products used for personal health or cosmetic reasons. This category includes a broad group of chemical substances such as human and veterinary medicines and cosmetics. The presence of PPCPs in environmental and potable water is a widespread concern due to the potentially harmful environmental effects. Evidence suggests PPCPs are linked to some ecological damage such as delayed development in fish (1).

To ensure the safety of water, PPCP concentrations are stringently monitored by environmental regulatory bodies, including the United States Environmental Protection Agency (US EPA) (2). Detection of PPCPs is traditionally a complicated process due to the range of substances potentially present. Here we explore a simple, more convenient method than traditional solid-phase extraction (SPE)-based methods for highly sensitive PPCP detection, using triple quadrupole liquid chromatography–mass spectrometry (LC–MS–MS).

Detecting PPCPs


Figure 1: PPCPs in environmental water and nearby soil is a widespread concern.
Conventional methods of PPCP detection in clean water have followed the defined EPA 1694 "template" for analysis, which requires the pre-concentration of large volume water samples and tedious solid-phase extraction cleanup, followed by liquid chromatography–mass spectrometry analysis to achieve the low ng/L (ppt) level detection necessary to comply with regulations (3).


Figure 2: Selected MRM chromatograms for PPCPs at 2 ppt.
Bruker has explored how LC–MS–MS can be employed specifically for the analysis of PPCPs in clean water. PPCPs were detected at 1–2 ppt with a linear response up to 200 or 500 ppt. Excellent system robustness was obtained throughout the extended method development and sample analysis period.

Case Study: Using LC–MS–MS to Analyze PPCPs in Clean Water


Figure 3: Selected calibration curves.
The study was carried out using ultrahigh-performance liquid chromatography (UHPLC) with an integrated on-line extraction (OLE) option coupled to a triple quadrupole mass spectrometer. The OLE module enables convenient method-driven on-line sample cleanup or sample pre-concentration.


Tables 1a & 1b: Instrumentation set-up for analysis of PPCPs in clean water.
Several water samples were analyzed for a range of PPCP species, including tap water samples along with bottled water and creek water. Samples were analyzed targeting a wide range of PPCP species representing compounds displaying varied properties and concentrations. Tables 1a and 1b illustrate the Advance UHPLC and EVOQ instrumentation set up respectively.


Table 2: Test results for selected PPCPs in real water samples.
All of the PPCPs were detected at 2 ppt or better with the injection of 0.4 mL samples with a linear response range up to 200 or 500 ppt. The fast polarity switch can analyze positive and negative PPCPs in the same analytical segment with excellent linear response for both polarities (Figures 2 and 3). The results for the analysis of tap, creek, and bottled waters are shown in Table 2.

Conclusion

The Bruker Advance UHPLC with OLE coupled to EVOQ LC–MS–MS detected PPCP samples at 2 ppt or better within 0.4 mL samples. Excellent linearity, sensitivity, and robustness were achieved throughout. The technique presents a more convenient and simpler approach to PPCP analysis than traditional SPE-based methods.

References

(1) K. Hirsch, "Pharmaceuticals and Personal Care Products," (2013). Available at: http://serc.carleton.edu/NAGTWorkshops/health/case_studies/pharmaceutical.html.

(2) US EPA, "PPCPs Basic Information," (2010). Available at: http://www.epa.gov/ppcp/basic2.html.

(3) US EPA, "EPA Method 1694: Pharmaceuticals and Personal Care Products in Water, Soil, Sediment, and Biosolids by HPLC/MS/MS," (2007).


Bruker Daltonics Inc.
40 Manning Road, Billerica, Massachusetts 01821, USA
Tel: (978) 663 3660 Fax: (978) 667 5993
Website: http://www.bruker.com/

ADVERTISEMENT

blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:




 

LCGC COLUMNISTS 2014

Column Watch | Ronald E. Majors: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: Standardized Testing of Silica as a Base Material for Difficult Bonded-Phase Preparative Applications


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Seven Common Faux Pas in Modern HPLC


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Retention Time Variation Is Normal?


More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>


Source: The Application Notebook,
Click here