The update to the SANCO guidelines in 2009 recognized the power and potential of TOF mass spectrometry for pesticide screening
applications. When compared with historical screening tools (such as the use of triple-quad mass spectrometry), high performance
accurate mass TOF systems offer several advantages for screening applications. These advantages include better resolution,
unlimited multiple target capabilities, and the possibility to perform comprehensive and/or retrospective analysis on samples
in silico at will.
The maXis impact ™ continues the evolution of Bruker's unique TOF accurate mass instruments. The performance of ≥40k resolution,
≤ 1ppm mass accuracy, and high isotopic fidelity is maintained without sensitivity compromise. While acquisition at up to
50 Hz enables maXis impact to handle the fastest of UHPLC separations with ease. Additionally, to aid in quantitative applications,
the maXis impact delivers an increase in sensitivity of up to 50-fold over previous generation instruments, especially at
the low m/z MS-MS signals important for analyte confirmation.
Figure 1: Fluoxastrobin at 50 fg to 1 ng on column.
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The dramatic increase in sensitivity changes the game for pesticide screening applications. With LODs typically in the sub
ng/mL levels, achieving excellent MRLs becomes very easy. The maXis impact enables analysts to obtain both qualitative and
quantitative results in a single analysis. Identification data including accurate mass MS and MS-MS, isotopic pattern fitting
and Rt fitting can be readily collected and correlated in dedicated software. In the same run, sub ng/mL LOQs with dynamic
ranges over four orders of magnitude can also be obtained (Figure 1).
Taken together, the key qualitative and quantitative performance attributes of the maXis impact make it an excellent choice
for routine pesticide screening applications with confirmation and quantitation in a single run. The maXis impact can provide
comprehensive data quality that is unmatched by any other technology currently available.
Experimental
A mixture of 200 pesticides was run on the PesticideScreener™ application. This mixture has previously been well characterized
and publicized. This same mixture was used to assess the performance of the maXis impact and specifically to assess the increase
in sensitivity observed.
Conclusions
Figure 2: Overlapped hrEIC traces showing the evolution of TOF sensitivity.
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The maXis impact changes the game in small molecule pesticide screening. Sensitivity increases of 20- to 50-fold versus previous
systems are routine. This enhanced sensitivity, coupled with the additional capabilities of the maXis impact, allow both high
quality qualitative and quantitative data to be acquired in a single run (Figure 1). The capabilities of the maXis impact
are ideally suited to pesticide screening and quantification, and provide an unrivalled level of confidence at sub ppb levels.
To further optimize the use of the maXis impact for pesticide screening, the PesticideScreener application platform is a fully
developed protocol, allowing the use of accurate mass and isotopic pattern fitting and retention time fitting, for straight-out-of-the-box
implementation of this outstanding analytical system (Figure 2).
The maXis impact sets the new gold standard for pesticide screening through full utilization of the capabilities of ultrahigh-sensitivity
TOF accurate mass spectrometry.
Bruker Daltonics Inc.
40 Manning Road, Billerica, MA
tel. (978) 663-3660, fax (978) 667-5993
Website:
http://www.bruker.com/
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