Basic instinct - - Chromatography Online
Basic instinct

The Column

Spodoptera littoralis is a destructive caterpillar pest indigenous to tropical and subtropical areas of agriculture that has the potential, if accidentally imported, of becoming a major greenhouse pest in Northern Europe. The UK government agency Defra reports that many populations of the species are already highly pesticide resistant2.

S. littoralis possess a pair of antennae for sensory perception, that vary in morphology between the larval and adult stages of development. Up until now, it was thought that on maturation, only adult males expressed specific protein receptors for the detection of female sex pheromones. Scientists at the Insect Physiology: Signalling and Communication research unit in Versailles, France 1, have identified that S. littoralis respond to the treatment of food sources with adult sex pheromone, providing an unexpected cue for food detection in S. littoralis larvae.

Gas chromatography–mass spectrometry (GC–MS) was utilized to extract the key component of S. littoralis pheromones from two-day old (adult) S. littoralis. Choice tests demonstrated that the larvae have an undefined mechanism of sensing adult pheromone that acts as a cue for food detection.

“Since the caterpillar behaviour we evidenced was totally unexpected, we had to use a combination of different approaches to be sure of it,” commented Dr Jacquin-Joly, leader of the team. “Gas chromatography coupled to mass spectrometry represents an invaluable tool to identify and quantify key odorant components in a natural extract.”

1. Emmanuelle Jacquin-Joly et al, Nature Comm. 3:1047 (2012).

2. Defra. “African Cotton Leafworm, Spodoptera littoralis: Fact-sheet”, (www. secure.fera.defra.gov.uk- Accessed September 2012).

This story originally appeared in The Column. Click here to view that issue.

ADVERTISEMENT

blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:




 

LCGC COLUMNISTS 2014

Column Watch | Ronald E. Majors: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: Standardized Testing of Silica as a Base Material for Difficult Bonded-Phase Preparative Applications


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Seven Common Faux Pas in Modern HPLC


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Retention Time Variation Is Normal?


More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>


Source: The Column,
Click here