CUSTODION ™ SPME Syringe for Rapid Sample Collection and Sample Preparation of Drinking Water for the GC–MS Determination of Trihalomethanes - - Chromatography Online
CUSTODION ™ SPME Syringe for Rapid Sample Collection and Sample Preparation of Drinking Water for the GC–MS Determination of Trihalomethanes


The Application Notebook

Douglas W. Later, Edgar D. Lee, Joseph L. Oliphant, and Christopher R. Bowerbank Torion® Technologies Inc.

The CUSTODION™ SPME Syringes are a series of novel solid phase micro extraction syringes that incorporate Supelco Analytical's® (Bellefonte, Pennsylvania) SPME fiber technology. The SPME syringes are fabricated with injection-molded components and the device resembles a ball-point pen. The Supelco Analytical® SPME fiber assembly is housed inside the syringe.


Figure 1
The push button trigger on top of the syringe (see Figure 1) enables the SPME fiber to be withdrawn into a protective metal sheath after sampling and while being inserted into the heated injection port of a GC or GC–MS. Target analytes in air, headspace, liquid, or dissolved solid samples are quickly trapped on the active coated surface of the SPME fiber, which is mounted inside the easy-to-operate syringe. The CUSTODION SPME syringes are designed for easy singlehanded operation when wearing protective gloves.

Experimental Conditions

A divinylbenzene/polydimethylsiloxane (DVB/PDMS, 65 μm df) SPME phase was exposed to the headspace above drinking water samples for replicate 1 min intervals following sample agitation between each sampling, for a total of 5 min sampling time. Concentrations of 10, 20, 100, and 500 ppb for each of the four trihalomethanes (THMs) were tested in this manner with freshly prepared standards from a 2000 ppm stock solution. The analysis was performed on a GUARDION™ -7 GC–TMS system.

  • Matrix: Drinking water
  • Analytical column: MTX-5 (5 m × 100 μm I.D., 0.25 μm df)
  • GC Temperature Conditions: 40–280°C, 2°C/s
  • Scan range: 50 –400 m/z
  • Carrier gas: Ultra-High Purity (UHP) Helium
  • Detector: Toroidal ion trap mass spectrometer (TMS)

Results


Figure 2
Figure 2 shows the GC–TMS separation of trihalomethanes spiked into a drinking water sample. All four THMs were detected and positively identified by the GUARDION-7 compound library at 100 and 10 ppb. The CUSTODION SPME syringe fiber was used to collect only the headspace of the drinking water sample and was not exposed directly to the water.

Conclusions

The CUSTODION SPME syringes provide a quick, easy, and reliable technique for sample collection and preparation. The trigger button activation of the SPME holder allows for single handed operation during sampling and analysis. The EPA drinking water standard is 80 ppb total cumulative concentration for the four THMs. At detection levels for the individual THMs of ~10 ppb, as shown here, this method can be easily used to determine compliance of drinking water samples in the field at the point source in less than 5 min.

Acknowledgements

CUSTODION™ and GUARDION™ are registered trademarks of Torion® Technologies Inc. CUSTODION SPME Syringes are manufactured and sold under license from (1) Supleco Analytical® under U.S. Patent 5,691,206 and (2) Brigham Young University under U.S. Patent Application 11/379,716 and/or any divisions, continuations or revisions thereof.




Torion® Technologies Inc.
796 East Utah Valley Drive, Suite 200,
American Fork, Utah 84003
http://www.torion.com/

ADVERTISEMENT

blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:




 

LCGC COLUMNISTS 2014

Sample Prep Perspectives | Ronald E. Majors:

LCGC Columnist Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments in existing technology lines.

LATEST: The Role of Selectivity in Extractions: A Case Study

History of Chromatography | Industry Veterans:

With each installment of this column, a different industry veteran covers an aspect of the evolution and continued development of the science of chromatography, from its birth to its eventual growth into the high-powered industry we see today.

LATEST: Georges Guiochon: Separation Science Innovator

MS — The Practical Art| Kate Yu:
Kate Yu is the editor of 'MS-The Practical Art' bringing her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers.

LATEST: Mass Spectrometry for Natural Products Research: Challenges, Pitfalls, and Opportunities


LC Troubleshooting | John Dolan:

LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan, Vice President of LC Resources and world renowned expert on HPLC, is able to highlight common problems and provide remedies for them.

LATEST: LC Method Scaling, Part I: Isocratic Separations

More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>


Source: The Application Notebook,
Click here