Chromatography at the National Chromatographic R&A Center, Dalian Institute of Chemical Physics
The major research interest of this high-efficiency separation and high-sensitivity characterization group is focused on the
development of novel separation and detection techniques for proteome analysis. To achieve this goal, research on the following
aspects has been performed:
1. Synthesis of monolithic materials. Monolithic materials have been regarded as a new generation of chromatographic stationary phases, offering the advantages
of fast mass transfer, low backpressure and ease of modification. The group aims to synthesize various novel monolithic materials
for the on-line selective concentration of phosphorated peptides by IMAC,1 the deleption of high-abundance proteins and enrichment of low-abundance proteins by MIPs, the development of immobilized
enzymatic reactor2 and the synthesis of novel separation matrices for HPLC, CEC and immobilized pH gradient (IPG) for CIEF.3 2. Multidimensional separation platforms. To meet the greater and greater demands of proteome analysis, their emphasis is on the construction of different multidimensional
separation platforms, including 2D-HPLC, 2D-CE4,5 and microchip 2D-CE. Consequently, optimization of separation conditions, design of 2D separation interfaces and preparation
of novel stationary phases for microchip-based separations, form the basis of their studies. 3. On-line hyphenation with MS/MS. To achieve high sensitivity and high accuracy identification of proteins, their research is aimed at the development of various
hyphenation techniques of HPLC, IPG-CIEF, CEC and pCEC6 with MS, with emphasis on the improvement of the compatibility of separation and identification systems.
For more information, contact Professor Lihua Zhang on e-mail: lihuazhang@dicp.ac.cn
or visit http://www.proteomics.dicp.ac.cn/en_index.htm
References
1. Lingyi Zhang et al., Electrophoresis, 26, 2172–2178 (2005).
2. Jicheng Duan et al., Proteomics, 6, 412–419 (2006).
3. Guijie Zhu et al., Electrophoresis, 27, 3578–3583 (2006).
4. Hechun Liu et al., Anal. Chem., 76, 6506–6512 (2004).
5. Chun Yang et al., Anal. Chem., 75, 215–218 (2003).
6. Zhen Liang et al., Anal. Chem., 76, 6935–6940 (2004).
Bioanalysis at the National Chromatographic R&A Center, Dalian Institute of Chemical Physics
The major research interest of this group is method and technology development for analysis of biological samples.
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