Pesticides are widely used in agriculture to protect crops from insect infestation. An important application of this equipment
is the possibility to analyze pesticides in water without pretreatment of the sample. Shimadzu has developed a system for
injection of water samples using Nexera with LCMS-8030 including on-line solid-phase extraction (SPE).
Pesticides are widely used in agricultural products to protect crops from insect infestation during cultivation and product
transport. The abuse of pesticides can cause significant environmental pollution in many areas, especially at the level of
lakes, rivers, and aquifers. For this reason, one of the most important and attractive applications is the ability to analyze
pesticides in water without pretreatment of the sample. In cooperation with Arpa Asti (Italy), Shimadzu developed a system
for injection of water samples using a LCMS-8030 triple quadrupole in combination with a Nexera UHPLC instrument with on-line
solid-phase extraction (SPE) to identify a mixture of 48 substances.
Materials and Methods
A standard mixture of pesticides was used for optimization of all multiple reaction monitoring (MRM) parameters. A 1 mL amount
of previously filtered sample was injected directly and an on-line SPE column was applied for concentration. The design of
the system allows switching between different injection volumes within one instrument configuration, which is completely controlled
by the Shimadzu LabSolutions Software (Figure 1). With a single autosampler it is therefore possible to inject up to 50 µL
volume for conventional UHPLC analysis and up to 2 mL sample volume for the analysis with on-line SPE pre-concentration.
Figure 1: Schematic design and flowline diagram of the Shimadzu solution for an on-line SPE and conventional UHPLC analysis
within one system.
The Shimadzu LCMS-8030 was applied in MRM mode for detection of the researched compounds. For construction of a calibration
curve water samples spiked with a standard were used. Figure 2 shows two exemplary calibration curves of metamitron and cloridazon
from a total of 48 substances, all in a concentration range from 0.002 µg/L to 0.100 µg/L, and two different examples of real
sample injections using on-line SPE.
Figure 2: Calibration curves of metamitron and cloridazon and examples of real sample injection using on-line SPE.
In addition to the on-line SPE option with large volume injection for pesticides in water, the system can be used as an UHPLC
instrument for low volume injections applying the same autosampler to reduce costs and the number of systems.
The new LCMS-8050 — speed and sensivity beyond comparison.
The UFMS series (Ultra Fast Mass Spectrometry) from Shimadzu not only provides extremely high speed analysis but also impressive
high sensitivity, to meet the growing demand for trace-level quantitation in various application fields. The newest member
of the UFMS-family, the LCMS-8050 features the world's fastest data acquisition rates and best-in-class sensitivity. Method
transfer of other models (LCMS-8030 and LCMS-8040) can easily be adopted.
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