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LCGC TV Library


LCGC TV: Screening Environmental Pollutants in Water Samples
Anthony Gravell of Natural Resources Wales describes a recent study comparing passive sampling with spot sampling to determine pollutants (including PAHs) in drinking water zones in England and Wales. Watch Here >>

 


LCGC TV: Frederic Lynen on Performing UHPLC in Bioanalysis
Are mass spectrometers becoming too sensitive? Does performing ultrahigh-performance liquid chromatography (UHPLC) increase matrix effects? In this new video clip from LCGC TV, Frederic Lynen of Ghent University in Belgium discusses the challenges faced by analysts working in the field of bioanalysis and comments on potential solutions. Watch Here >>

 


LCGC TV: Michal Holčapek on Trends in LC–MS
In this new video from LCGC TV, Michal Holčapek from the University of Pardubice in the Czech Republic describes the requirements of "fast LC–MS" and areas where the technique can be applied in industry. Watch here>>

 


LCGC TV: 2D LC Methods for Analyzing Pharmaceutical Impurities
Two-dimensional liquid chromatography has moved from academia into industry. In this new video, Kelly Zhang of Genentech talks about the advantages gained by developing a two-dimensional LC method for analyzing impurities in pharmaceuticals, and gives practical examples. Watch here>>

 


LCGC TV: Is 2D LC Ready for Prime Time Analysis?
2D LC has huge potential to increase the resolving power of separations involving complex samples. In this new video clip, Dwight Stoll of Gustavus Adolphus College considers if this technique is ready to be adopted by chromatographers working in industry. Watch here>>

 


LCGC TV: Mary Ellen McNally on Maintaining Precision During Method Transfer
Transferring methods between laboratories can be extremely difficult. In this new short video from LCGC TV, Mary Ellen McNally from DuPont Crop Protection gives her advice on best practices for maintaining precision during method transfer. Watch here>>

 


LCGC TV: Richard Henry on Optimizing UHPLC Methods
Ultrahigh-performance liquid chromatography (UHPLC) offers improved run-times and enhanced resolution when compared with conventional liquid chromatography (LC) methods. In this new video from LCGC TV, Richard Henry, a consultant from State College (Pennsylvania, USA), gives his advice on how to select UHPLC column size and discusses the features of an optimal UHPLC instrument.

 


LCGC TV: Sample Preparation for Chromatographic Methods in Metabolomics
Advances in mass spectrometry instrumentation have opened up the field of metabolomics, but there are still issues with non-standardized sampling and sample preparation methods. Tuulia Hyötyläinen from the VTT Technical Research Centre of Finland discusses sample preparation techniques performed in metabolomics, and gives a brief overview of what to consider when choosing a sample preparation technique. Watch here>>

 


LCGC TV: Koen Sandra on The Importance of LC–MS in Developing Protein Biopharmaceuticals
By 2020, more than 50% of approved drugs are expected to be protein biopharmaceuticals. Koen Sandra from the Research Institute for Chromatography in Belgium, discusses the importance of LC–MS in the development of biosimilars as drug patents expire, and future avenues of research.Watch here>>

 


LCGC TV: Frederick Lynen on the Challenges of Developing a HILIC-based SPE Approach to Analyze Biological Fluids
A single drop of blood represents a complex mixture of biological compounds including phospholipids, proteins, and salts. Frederic Lynen of Ghent University in Belgium discusses the possibilities of a HILIC-based SPE approach to remove phospholipids from blood samples, its applicability to other organic molecules, and how it benefits the chromatographer in practice. Watch here>>

 


LCGC TV: Michael Holčapek on Recent Trends in LC–MS
Quantitation is of unrivalled importance in all areas of analytical sciences. In this new video from LCGC TV, Michal Holčapek from the University of Pardubice in the Czech Republic discusses how to select internal standards for quantitative mass spectrometry and whether or not the chromatography element will ever disappear from LC–MS.

Watch here>>

 


LCGC TV: Dierdre Cabooter on Method Development in UHPLC
The Kinetic Plot method is a useful tool in LC method development to compare the performance of different LC columns with each other. In this new video from LCGC TV, Deirdre Cabooter from KU Leuven (Belgium) describes the range of techniques that it can be applied to and how it can help analysts in practice.

Watch here>>

 


LCGC TV: Richard Henry on Narrow Particle Size Distribution in HPLC Columns
Fully porous particle columns with narrow particle distribution can improve the efficiency of HPLC separations. In this new video from LCGC TV, Richard Henry, a consultant from State College (Pennsylvania, USA) discusses the advantages of narrow particle distribution and whether optimized LC instruments are required for optimum performance. Watch here>>

 


LCGC TV: Using the Variable Length Method Development Strategy to Develop Successful UHPLC Methods
The Kinetic Plot method is a useful tool in LC method development to compare the performance of different LC columns with each other. In this new video from LCGC TV, Deirdre Cabooter from KU Leuven (Belgium) describes how to use the variable length method development strategy to develop successful UHPLC methods and how this approach can save time for the analyst. Watch here>>

 


LCGC TV: Mary Ellen McNally on Analytical Precision
In these two new videos from LCGC TV, Mary Ellen McNally from DuPont Crop Protection addresses the issue of precision, covering the reasons why the precision of a measurement is so important and the factors that can influence the precision of an active ingredient assay.

 


LCGC TV: Why You Need to Listen . . . Even to Your Instrument
We all have always been told that we need to listen, and to listen more carefully. Growing up, we were told to listen to our parents and our teachers. As adults, we need to listen more attentively to our spouses at home and our bosses, colleagues, and customers at work. But have you considered listening to your analytical instruments? Here is why you should. Watch here>>

 


LCGC TV: Part II: Milton Lee on the Potential of Thermal Gradient Gas Chromatography (TGGC)
First developed in the 1960s to reduce peak broadening effects, thermal gradient gas chromatography (TGGC) is now being re-examined. In these three new videos from LCGC TV, Milton L. Lee from Brigham Young University discusses the application areas of TGGC, the “peak sweeping” approach, and whether or not the technique is ready for industry.

 


LCGC TV: What forensic labs are up against

Analysts in forensic labs work in a challenging landscape, where the targets of analysis keep changing. In two new short video clips, Tom Brettell explains how these challenges play out in terms of their use of GC columns and GC methods.

 


LCGC TV: Should you be using GCxGC in your routine analysis?

Two-dimensional gas chromatography (GCxGC) started out as a specialized technique reserved for the domain of academic researchers. But the technique, and the instrumentation, have evolved significantly in the past decade. Does it make sense to use GC x GC in routine analysis? Frank Dorman weighs in. Watch here>>

 


LCGC TV: How to Pick Column Combinations for GCxGC

When you are setting up a two-dimensional GC separation (GCxGC), how do you get the optimal combination of columns with the least amount of method development pain? In two new short videos, Nick Snow of Seton Hall offers advice, about column type and column length.

 


LCGC TV: Rethinking Sample Prep

When we asked Doug Raynie where he sees sample preparation heading, he replied not in terms of specific techniques, but instead in terms of the overarching considerations that we should keep in mind. Do we need to rethink sample prep? Watch here>>

 


LCGC TV: Sample Preparation for Chromatographic Methods in Metabolomics

Advances in mass spectrometry instrumentation have opened up the field of metabolomics, but there are still issues with non-standardized sampling and sample preparation methods. Tuulia Hyötyläinen from the VTT Technical Research Centre of Finland discusses the vast range of sample matrices and gives advice on best practices. Watch here>>

 


LCGC TV: Using Passive Sampling in Environmental Analysis

Anthony Gravell of Natural Resources Wales discusses the principles of passive sampling and the advantages of the technique over spot sampling when screening water samples for environmental contaminants. He also gives an overview of the challenges and considerations that have to be made when performing LC–MS on passive sampling extracts. Watch here>>

 


LCGC TV: What's Behind the Trends in Sample Preparation

There are some interesting shifts going on in sample preparation. In this new short video interview, Doug Raynie of South Dakota State University discusses what is behind the trends — starting with the fundamental differences in strategies for solid and liquid samples. Watch here>>

 


LCGC TV: Is Two-Dimensional LC Worth the Effort?

Two-dimensional liquid chromatography (2D LC) is a powerful technique, but method development is more involved and time-consuming. So for what types of analyses does it make sense to invest the extra effort to develop a 2D LC method instead of a 1D method? Dwight Stoll explains. Watch here>>

 


LCGC TV: Common Errors in the Laboratory

We all make mistakes. The first step to correcting them is recognizing them, and learning the right way to do things. In two new short videos, Merlin Bicking talks about two common errors that chromatographers make, and how to avoid them. Watch here>>

 


LCGC TV: The Future of Monoliths and Chips

Monoliths have received renewed attention in the past few years. Will they — or chip-based columns — ever compete with particle-based columns? In a short video interview, Dick Henry weighs in. Watch here>>

 


LCGC TV: Deirdre Cabooter on Method Development in UHPLC

Performing ultrahigh-performance liquid chromatography (UHPLC) can significantly reduce analysis time without compromising on efficiency by using sub 2-μm particles that allow increased velocity while maintaining selectivity. In this new short interview, Deirdre Cabooter of KU Leuven in Belgium, discusses the advantages of using UHPLC in method development and tips to follow when transferring methods from UHPLC to HPLC instrumentation. Watch here>>

 


LCGC TV: Two-Dimensional LC in Pharmaceutical Analysis: Advantages and Challenges

Two-dimensional liquid chromatography has moved from academia into industry. In two new short video interviews, Kelly Zhang of Genentech talks about the advantages gained by developing a two-dimensional LC method for analyzing impurities in pharmaceuticals, as well as the challenges in developing the method. Watch here>>

 


LCGC TV: GC in Forensic Laboratories

Tom Brettell, an associate professor of chemistry at Cedar Crest College, USA, recently conducted a survey of gas chromatography column use in forensic laboratories in the United States. Here is what he found, in terms of the types of columns they are using and the problems they face. Watch here>>

 


LCGC TV: The Potential of Thermal Gradient Gas Chromatography (TGGC)

Thermal gradient gas chromatography (TGGC) was first developed in the 1960s to reduce the effects of peak broadening. With recent developments in technology, the technique is now being re-examined. In two new videos from LCGC TV, Milton L. Lee from Brigham Young University discusses the principles of TGGC, and the possible advantages of TGGC over traditional GC methods. Watch here>>

 


LCGC TV: Analyzing Drugs of Abuse

In a recent study, Nick Snow of Seton Hall University studied the presence of drugs of abuse in various complex matrices to detect trace quantities of drugs of abuse such as salvorinin A. In a new short video, Snow explains why used GCxGC–TOF-MS is a good choice for this type of analysis. Watch here>>

 


LCGC TV: The Pros and Cons of GCxGC

Two-dimensional gas chromatography has advanced considerably since its first emergence in the late 1980s and its first applications for the hydrocarbon fingerprinting of crude oils. In a new interview, Frank L. Dorman of Penn State University talks about the evolution of GCxGC and the key advantages and disadvantages of the technique, including why fear of its complexity gets in the way of its broader use. Watch here>>

 


LCGC TV: Eliminating Matrix Effects: LC–MS Analysis of Biological Fluids

Frederic Lynen of Ghent University in Belgium discusses why LC–MS is the ideal analytical tool for the quantitative analysis of analytes in biological samples, and presents a new HILIC-based SPE approach to improve analyte recovery and sensitivity by removing phospholipids from samples. Watch here>>

 


LCGC TV: Richard Henry on Fully Porous vs. Superficially Porous Particles

Superficially porous particles for HPLC columns – often called "core–shell" particles have gotten a lot of attention in recent years because of their excellent performance. Yet so far, columns made with fully porous particles are still the most widely used. Will superficially porous particles eventually take over? Watch here>>

 


LCGC TV: Dwight Stoll on 2D LC

In this new video interview, Dwight Stoll of Gustavus Adolphus provides advice about key decisions in method development, common errors to avoid, and other advice for getting started with 2D LC. Watch here>>

 


LCGC TV: Richard Henry on "Do We Need More LC Phases?

Dick Henry, consultant, discusses a trend that could renew efforts towards developing novel LC stationary phases with improved selectivity, peak symmetry, and stability. The question is, however, whether we really need more stationary phases in HPLC. Watch the video and find out. Watch here>>

 


LCGC TV: Mary Ellen McNally on Common Error with Internal Standard Methods

Internal standards are an important tool of analytical chemists, yet errors in their usage are common. Mary Ellen McNally of DuPont Crop Protection offers advice for how to introduce an internal standard and how to avoid common errors with internal standard methods. Watch here>>

 


LCGC TV: Michal Holcapek on Trends in LC–MS

More and more analysts are moving away from low-resolution mass spectrometry to high-resolution mass spectrometry, but what are the requirements for making such a change? Michal Holcapek, professor of analytical chemistry at the University of Pardubice, Czech Republic, discusses what you need to consider when switching to a high resolution system. Holcapek also offers advice on how to choose the most effective ionization technique for your analysis. Watch here>>

 


LCGC TV: Koen Sandra on Analyzing Protein Biopharmaceuticals Using LC–MS

By 2020, more than 50% of approved drugs are expected to be protein biopharmaceuticals. Koen Sandra from the Research Institute for Chromatography in Belgium discusses the challenges of characterizing protein biopharmaceuticals and why LC–MS is now indispensable for characterizing this class of drugs. Watch here>>

 


LCGC TV: Joe Foley on Sequential Elution Liquid Chromatography

Sequential-elution LC is a new methodology for the analysis of complex samples. In a new video initiative from LCGC, Joe Foley, Professor of Chemistry at Drexel University, Philadelphia, Pennsylvania, USA, explains very briefly how it works and its advantages over conducting multiple runs of conventional LC. Watch here>>

 


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LCGC COLUMNISTS 2014

Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase Chemistry


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Seven Common Faux Pas in Modern HPLC


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: Estimating Resolution for Marginally Separated Peaks


More LCGC Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>



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