Tuesday, February 12, 2013

1:00 PM EST

Editors’ Series: HPLC of Very Polar Compounds in Bioanalysis

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There are two fundamental approaches that can provide the answers to how diseases function biochemically and a means for diagnosing them at an early stage. The first is to identify the proteins that are involved in the disease and the second is to monitor the chemical compounds (metabolites) produced by the disease as a result of human physiological processes. Generically, these two fields of study are referred to as proteomics and metabolomics. The presentation will focus on high performance liquid chromatography (HPLC) approaches related to analysis of polar small molecules that are classified as pharmaceuticals, metabolites, or biomarkers. Many of these are polar compounds that are often challenging for traditional reversed-phase separation methods. One approach for analyzing these types of samples is the use of columns having polar retention capabilities such as ion-exchange, hydrophilic interaction liquid chromatography (HILIC), polar embedded, or polar endcapped columns. Another approach is aqueous normal phase (ANP) chromatography. This mode has the advantage of having both reversed-phase and normal-phase retention. In addition, examples of challenging physiological and pharmaceutical applications using recently developed methods using MS detection will be presented.

Sponsored by: Agilent Technologies