Breath analysis - - Chromatography Online
Breath analysis

The Column
Volume 8, Issue 20

Steptococcus pneumoniae and Haemophilus influenzae are the causative agents of community-associated pneumonia (CAP). CAP is principally responsible for the highest number of deaths resulting from bacterial infection. The illness mainly infects young adults, children and those who are immunocompromised because of a pre‑existing condition, such as patients prescribed with immunosuppressant drugs following organ transplantation. This group of patients are therefore unlikely to be comfortable undergoing current diagnostic tests that are considered invasive and slow to the point that the infection can become more advanced. This increases the demand for a non-invasive, rapid and economic test that can pinpoint the presence of infection before it can progress.

A group of scientists in Austria 1 have developed and characterised a method for the detection of S. pneumoniae and H. influenzae in the upper respiratory tract by analysing breath samples of patients. Using gas chromatography coupled to mass spectrometry (GC–MS), the investigators identified a broad spectrum of volatile organic compounds (VOCs) associated with the two infections. Headspace samples were adsorbed to multi‑bed sorption tubes prior to GC–MS analysis. The analysis resulted in the identification of 34 VOCs released from S. pneumoniae and 28 VOCs released from H. influenzae and interestingly for S. pneumoniae catabolism of aldehydes was detected.

These initial findings of differential chemical profiles associated with the two different respiratory infections indicate that there is the opportunity for the development of a portable breath gas analyser that may be less invasive and intrusive than current diagnostic methods. Further investigations are required to increase the sample number of patients and to eliminate patient variability.

1. A. Ammann et al, Microbiology, DOI: 10.1099/mic.0.0626870 (2012).

This story originally appeared in The Column. Click here to view that issue.


blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:



Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: When Bad Things Happen to Good Food: Applications of HPLC to Detect Food Adulteration

Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: HPLC for Characterization and Quality Control of Therapeutic Monoclonal Antibodies

MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis

LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Can I Inject? Part I: Injecting in Mobile Phase

More LCGC Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>

Source: The Column,
Click here