Healthy ham - - Chromatography Online
Healthy ham

The Column
Volume 8, Issue 22

A collaborating team of investigators1 based in Spain, Japan and the UK believe they may have discovered the potential health benefits of Spanish dry-cured ham. They consider that the ham could reduce high blood pressure associated with cardiovascular disease when consumed as part of a healthy diet.

The investigators fractionated water-soluble peptides from the ham samples taken from the ham by size-exclusion chromatography (SEC) followed by reversed-phase high performance liquid chromatography (RP-HPLC). Nano-liquid chromatography coupled to mass spectrometry was used to analyse a total of 73 peptides selected from bioactive fractions. The anti‑hypertensitivity of candidate peptides was quantified; notably, the majority of the candidates were of skeletal muscle origin. The in vitro activity of peptides was measured by testing inhibition of the enzymatic activity of angiotensin I converting enzyme (ACE). ACE is a common target for clinical inhibitors used for reducing hypertension. The in vivo hypertensive activity was measured by observing the effect of administering candidate peptides to hypertensive rats.

The unique peptide profile identified is hypothesized to be the result of an accumulation of fragment by-products of endogenous muscle enzyme break-down during the treatment of the ham that would otherwise not be present.

The team’s results suggest that the presence of the anti‑hypertensive peptides may off-set the adverse effects of the high levels of sodium chloride on blood pressure. Moreover, studies to elucidate the mechanism of action may lead to advances in drug discovery and development.

1. E. Escudero et al, Journal of Proteomics, ( (2012).

This story originally appeared in The Column. Click here to view that issue.


blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:



Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: When Bad Things Happen to Good Food: Applications of HPLC to Detect Food Adulteration

Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: HPLC for Characterization and Quality Control of Therapeutic Monoclonal Antibodies

MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis

LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Can I Inject? Part I: Injecting in Mobile Phase

More LCGC Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>

Source: The Column,
Click here