Silicone Protheses for Biomonitoring POPs in Humans - - Chromatography Online
Silicone Protheses for Biomonitoring POPs in Humans

The Column
Volume 9, Issue 19, pp. 6

Scientists from the Norsk Instituutt for Vannforsking (Norwegian Institute for Water Research), Oslo, Norway, have proposed the analysis of silicone explants for the monitoring of persistent organic pollutants (POPs) in humans.1 The team suggest the formation of repositories for discarded silicone implants from the cosmetic surgery industry.

So, where did this idea come from? Lead author Ian J. Allan told The Column that his team has been developing passive sampling methods using silicone to measure environmental POPs for over 10 years. In a recent trial study, the team used a silicone tag in a living fish to measure the contaminants in the river.2 Analysis of the tag showed that levels of POPs were comparable to the levels in the river water, therefore providing proof of concept. Allan said: “Directly comparing concentrations in biota (in units of ng per gram lipids) with concentrations in abiotic compartments, for example in water, (pg/ ng per litre) is difficult. The use of the same polymer for sampling different environmental compartments allows the comparison of the activity or fugacity of chemicals in these compartments more simply.”

Currently, human biomonitoring of POPs is generally performed using blood, milk, and adipose tissues — the World Health Organization (WHO) and Stockholm Convention are currently working together to survey global human exposure. These samples are difficult and expensive to obtain, whereas explanted silicone from humans is readily available. The paper quotes that an estimated 5–10 million women worldwide are implanted with silicone breast protheses, with 300,000 breast augmentations performed in the USA in 2010 alone. As implants have a life-span of 10–15 years, they have to be explanted and are then disposed of.

Allan said: “In vitro tissue exposure with passive sampling devices and in vivo methods in clinical settings (for example, with solid-phase microextraction fibres) have received increased attention in recent years and following discussions with my colleagues we realized that it may be possible to use explanted silicone prostheses as passive sampling devices for human biomonitoring.”

With the co-operation and help of a plastic surgeon based in Oslo, Norway, 33 explanted protheses were collected from 22 female clinics undergoing surgery at a clinic in Norway. Acetone was used to extract whole samples — according to Allan, acetone was selected because of the limited effect it has on the swelling of the silicone during extraction compared to nonpolar pentane or hexane. Gas chromatography coupled to mass spectrometry (GC–MS) was subsequently performed to detect organochlorides, polychlorinated biphenyls, and polybrominated diphenyl ethers.

The samples required concentrated sulphuric acid clean-up before GC–MS analysis for acid-resistant chlorinated and brominated substances, limiting the number of compounds that could be detected and investigated. Allan told The Column that the team are now currently working on sample clean-up methods that eliminate this step, and therefore increase the number of chemicals that can be sampled and quantified. The advantage of the method is that samples can be taken from different people but still be directly compared. Allan told The Column: “Ultimately we would like to combine this sampling with non-targeted screening analytical methods.”

The team will be working on developing the study to determine the application of the method to a wider range of silicone samples produced by different manufacturers, in addition to optimizing the method to reduce the level of solvents used and to increase the number of contaminants detectable. — B.D.


1. I.J. Allan et al. Environment International 59, 462– 468 (2013).

2. I.J. Allan et al. Environ. Sci. Technol. DOI: 10.1021/ es401810r (2013)


blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:



Sample Prep Perspectives | Ronald E. Majors:

LCGC Columnist Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments in existing technology lines.

LATEST: The Role of Selectivity in Extractions: A Case Study

History of Chromatography | Industry Veterans:

With each installment of this column, a different industry veteran covers an aspect of the evolution and continued development of the science of chromatography, from its birth to its eventual growth into the high-powered industry we see today.

LATEST: Georges Guiochon: Separation Science Innovator

MS — The Practical Art| Kate Yu:
Kate Yu is the editor of 'MS-The Practical Art' bringing her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers.

LATEST: Mass Spectrometry for Natural Products Research: Challenges, Pitfalls, and Opportunities

LC Troubleshooting | John Dolan:

LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan, Vice President of LC Resources and world renowned expert on HPLC, is able to highlight common problems and provide remedies for them.

LATEST: LC Method Scaling, Part I: Isocratic Separations

More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>

Source: The Column,
Click here