Recent Developments in Ion Mobility Mass Spectrometry for Bioanalysis - - Chromatography Online
Recent Developments in Ion Mobility Mass Spectrometry for Bioanalysis

The Column
Volume 10, Issue 15, pp. 1417

This article reviews recent developments that aim to address the conflicting demands for higher throughput, sensitivity, and selectivity in bioanalysis applications.

Photo Credit: Rafe Swan/Getty Images
Bioanalysis is a key part of a candidate drug's pharmacokinetic and pharmacodynamic characterization. Liquid chromatography coupled with tandem mass spectrometry (LC–MS–MS) has been used widely by pharmaceutical laboratories over the past 30 years to quantify drugs and their metabolites in biological matrices. Numerous advances in instrumentation, technology, methods, and software during this period have led to significant improvements in speed, and sensitivity alone has increased by seven orders of magnitude.1

Analyzing drugs in biological samples has always been challenging as a result of matrix effects or background interference, and researchers have tried to overcome this in the past with various method developments and sample preparation steps. More recently, addressing the issue has been complicated further by the need to increase sample analysis throughput, while at the same time being able to detect compounds at increasingly lower levels.

One approach has been to use ion mobility mass spectrometry (IMS), which combines ion separation techniques with mass spectrometry, including quadrupole or time-of-flight (TOF). IMS has been commonly used for the selective detection of compounds in biological samples.2,3 Ion mobility can operate at atmospheric pressure, unlike mass spectrometry, and this allows the incorporation of atmospheric pressure ionization.3 This enables additional selectivity during sample introduction, but IMS is typically too slow and not sufficiently rugged for quantitative bioanalysis applications.4


blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:



Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: When Bad Things Happen to Good Food: Applications of HPLC to Detect Food Adulteration

Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: HPLC for Characterization and Quality Control of Therapeutic Monoclonal Antibodies

MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis

LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Can I Inject? Part I: Injecting in Mobile Phase

More LCGC Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>

Source: The Column,
Click here