Optimizing Chromatographic Conditions for Protein Analysis with the
ACQUITY UPLC H-Class Bio System
Across the pharmaceutical industry, there is growing emphasis on the development of biopharmaceuticals as the next wave of drugs contributing to human health. In this podcast series, we’ll be discussing how chromatographic analyses of biopharmaceuticals, particularly proteins, compare to the more familiar assays of small molecule drugs and the benefits that UPLC® Technology can offer. The types of assays will be described and the basis for choosing them is considered. We’ll also discuss recent developments in ways to improve an analytical method.
The Importance of Multiple Chromatographic Modes for Protein Analysis
Biopharmaceutical proteins are large, complicated molecules and demand multiple modes of analysis to ensure complete characterization of the sample. The role of each analytical choice, in the characterization of a biomolecule, will be described as well as how the ACQUITY UPLC® H-Class Bio was designed to meet these analytical challenges.
Thomas E. Wheat
Milford, MA, USA
Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Seven Common Faux Pas in Modern HPLC