Podcast: The Importance of Multiple Chromatographic Modes for Protein Analysis - - Chromatography Online
LCGC Podcasts | A service of Chromatographyonline

Optimizing Chromatographic Conditions for Protein Analysis with the ACQUITY UPLC H-Class Bio System

Presented By

Across the pharmaceutical industry, there is growing emphasis on the development of biopharmaceuticals as the next wave of drugs contributing to human health. In this podcast series, we’ll be discussing how chromatographic analyses of biopharmaceuticals, particularly proteins, compare to the more familiar assays of small molecule drugs and the benefits that UPLC® Technology can offer. The types of assays will be described and the basis for choosing them is considered. We’ll also discuss recent developments in ways to improve an analytical method.

The Importance of Multiple Chromatographic Modes for Protein Analysis
Biopharmaceutical proteins are large, complicated molecules and demand multiple modes of analysis to ensure complete characterization of the sample. The role of each analytical choice, in the characterization of a biomolecule, will be described as well as how the ACQUITY UPLC® H-Class Bio was designed to meet these analytical challenges.

Presenter:
Thomas E. Wheat
Principal Scientist
Systems Laboratory
Waters Corporation
Milford, MA, USA




ONLINE RESOURCES



Add this Podcast feed to your media player



post a comment
Your email address will NOT be published.
appears with your comment
read our privacy policy
Note: does not support HTML
All Comments submitted are subject to review, and may be delayed before posting. Comments will be posted at the discretion of the editor.
LCGC E-mail Newsletters
Global E-newsletters subscribe here:




 
Survey
CHROMacademy
Are you aware of CHROMacademy and the learning tools it offers?
Yes, I would like to know more
Yes, I am not interested
No, I would like to know more
No, I am not interested
Yes, I would like to know more
69%
Yes, I am not interested
10%
No, I would like to know more
15%
No, I am not interested
6%
View Results

LCGC COLUMNISTS 2014

Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: When Bad Things Happen to Good Food: Applications of HPLC to Detect Food Adulteration


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: HPLC for Characterization and Quality Control of Therapeutic Monoclonal Antibodies


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Can I Inject? Part I: Injecting in Mobile Phase


More LCGC Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>



Click here