Optimizing Chromatographic Conditions for Protein Analysis with the
ACQUITY UPLC H-Class Bio System
Across the pharmaceutical industry, there is growing emphasis on the development of biopharmaceuticals as the next wave of drugs contributing to human health. In this podcast series, we’ll be discussing how chromatographic analyses of biopharmaceuticals, particularly proteins, compare to the more familiar assays of small molecule drugs and the benefits that UPLC® Technology can offer. The types of assays will be described and the basis for choosing them is considered. We’ll also discuss recent developments in ways to improve an analytical method.
The Importance of Multiple Chromatographic Modes for Protein Analysis
Biopharmaceutical proteins are large, complicated molecules and demand multiple modes of analysis to ensure complete characterization of the sample. The role of each analytical choice, in the characterization of a biomolecule, will be described as well as how the ACQUITY UPLC® H-Class Bio was designed to meet these analytical challenges.
Thomas E. Wheat
Milford, MA, USA
How useful do you find attending events such as HPLC 2013 for your research?
Not very useful
LCGC COLUMNISTS 2013
Column Watch | Ronald E. Majors:
<i>LCGC</i> Columnist Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments in existing technology lines.
History of Chromatography | Industry Veterans: With each installment of this column, a different industry veteran covers an aspect of the evolution and continued development of the science of chromatography, from its birth to its eventual growth into the high-powered industry we see today.
MS — The Practical Art | Kate Yu:
Kate Yu is the editor of 'MS-The Practical Art' bringing her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers.
LC Troubleshooting | John Dolan: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan, Vice President of LC Resources and world renowned expert on HPLC, is able to highlight common problems and provide remedies for them.