Waters Podcast series:Optimizing Chromatographic Conditions for Protein Analysis with the ACQUITY UPLC H-Class Bio System - - Chromatography Online
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Optimizing Chromatographic Conditions for Protein Analysis with the ACQUITY UPLC H-Class Bio System

Presented By Waters

With the increasing emphasis in biological drugs across the pharmaceutical industry, there is a growing interest in understanding and learning about the analytical methods that are required and the chromatographic choices that are needed. This is the first in a series of podcasts that discuss the chromatographic analysis of biopharmaceuticals, the benefits UPLC Technology brings to these applications, enabling you to get better quality data, faster, so you know more about your molecule.

Episode 4: Controlling the Impact of Chromatographic Mobile Phase Manipulation on Detection with UV and MS Detectors
Biopharmaceutical proteins are large, complicated molecules and demand multiple modes of analysis to ensure complete characterization of the sample. The choices in developing a separation method interact with detection, particularly in LC/MS. We will describe an experimental approach to optimizing this compromise.

Episode 3: Developing ion-exchange methods for protein analysis
This is the third in a series of podcasts that discuss the chromatographic analysis of biopharmaceuticals, the benefits UPLC technology brings to these applications, enabling you to get better quality data, faster, so you know more about your molecule

Episode 2: Controlling Mobile Phase pH for Biochromatography
Biopharmaceutical proteins are often best analyzed using ion exchange chromatography. Optimization of the methods has become much more efficient with UPLC Technology. In addition, to the characteristic high resolution separations, the ACQUITY UPLC® H-Class Bio System incorporates Auto●Blend Plus™ Technology for direct adjustment of pH and ionic strength.

Episode 1: The Importance of Multiple Chromatographic Modes for Protein Analysis
Biopharmaceutical proteins are large, complicated molecules and demand multiple modes of analysis to ensure complete characterization of the sample. The role of each analytical choice, in the characterization of a biomolecule, will be described as well as how the ACQUITY UPLC® H-Class Bio was designed to meet these analytical challenges.

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