The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part I: Background, Theory, and Potential Uses - - Chromatography Online
The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part I: Background, Theory, and Potential Uses


LCGC North America
Volume 30, Issue 9, pp. 842-849

References

(1) Wikipedia, http://en.wikipedia.org/wiki/Multiangle_light_scattering.

(2) Laser Light Scattering: http://www.ap-lab.com/light_scattering.htm.

(3) I.S. Krull, H. Stuting, and S. Kryzsko, J. Chromatogr. 442, 29 (1988).

(4) I.S. Krull, R. Mhatre, and H.H. Stuting, Trends in Anal. Chem. 8(7), 260 (1989).

(5) H.H. Stuting, I.S. Krull, R. Mhatre, S. Krzysko, and H. Barth, LCGC North Amer. 7(5), 402 (1989).

(6) R. Mhatre, H.H. Stuting, and I.S. Krull, J. Chromatogr. 502(1), 21 (1990).

(7) H.H. Stuting and I.S. Krull, Anal. Chem. 62, 2107 (1990).

(8) R. Mhatre and I.S. Krull, J. Chromatogr. 591, 139 (1992).

(9) R. Mhatre and I.S. Krull, Chromatographia 34, 357 (1992).

(10) D.J. Mageira and I.S. Krull, J. Chromatogr. 606, 264 (1992).

(11) R. Mhatre and I.S. Krull, Anal. Chem. 65, 283 (1993).

(12) R. Mhatre, R.-L. Qian, I.S. Krull, S. Gadam, and S. Cramer, Chromatographia 38(5/6), 349 (1994).

(13) I.S. Krull, R. Mhatre, and J. Cunniff, LCGC North Amer. 13, 30 (1995).

(14) M.E. Szulc, R. Mhatre, J. Mazzeo, and I.S. Krull, in High Resolution Separation of Biological Macromolecules, Methods in Enzymology Series, B.L. Karger and W. Hancock, Eds. (Academic Press, Waltham, Massachusetts, 1996), Chapter 8, p. 175.

(15) R.-L. Qian, R. Mhatre, and I.S. Krull, J. Chromatogr. A 787, 101 (1997).

(16) L.C. Santora, P. Sakorafas, Z. Kaymakcalan, I.S. Krull, and K. Grant, Anal. Biochem. 299(2), 119 (2001).

(17) Protein Pegylation Application Note, Protein Solutions, Wyatt Technology, Santa Barbara, California, 1996.

(18) Pegylated Protein Modifications Application Notes, Protein Solutions, Wyatt Technology, Santa Barbara, California, 1997.

(19) T.P. Second, A.W. Carr, R.C. Cummins, R. Viner, and L. Huang, "Characterization of PEGylated Peptides and Site Localization of Attachment with High Resolution ETD Mass Spectrometry," Thermo Fisher Scientific, Fremont, California, Application Note (2010).

(20) I.S. Krull, R. Mhatre, and J. Cunniff, LCGC North Amer. 12(12), 914 (1994).

(21) M.E. Szulc and I.S. Krull, in Practical HPLC Method Development, Second Edition, L. Snyder, J. Glajch, and J.J. Kirkland, Eds. (J. Wiley & Sons, Inc., New York, New York, 1997), Chapter 3.

(22) M.E. Szulc, R. Mhatre, J. Mazzeo, and I.S. Krull, in High Resolution Separation of Biological Macromolecules, Methods in Enzymology Series, B.L. Karger and W. Hancock, Eds. (Academic Press, Waltham, Massachusetts, 1996), Chapter 8, p. 175.

(23) C. Laue and D. Hunkeler, Int. J. Polym. Anal. Charact. 5, 511–529 (2000).

(24) T. Wang and J.A. Lucey, J. Dairy Sci. 86(10), 3090–3101 (2003).

(25) A. Oliva, M. Llabres, and J.B. Farina, Curr. Drug Discov. Technol. 1(3), 229–242 (2004).

(26) B.H. Zimm, J. Chem. Phys. 16, 1099–1116 (1948).

(27) P.J. Wyatt, Appl. Opt. 7(10), 1879–1896 (1968).

(28) W. Kaye and J.B. McDaniel, Appl. Opt. 13(8), 1934–1937 (1974).

(29) W. Schartl, Light Scattering from Polymer Solutions and Nanoparticle Dispersions (Springer-Verlag, Berlin, Germany, 2010).

(30) Absolute Characterization of Proteins and Biopolymers: Combining SEC with Light Scattering Detection, S.C. Kuebler, Wyatt Corporation Webinar, online at http://www.wyatt.com/, Figure 7.

(31) Absolute Characterization of Proteins and Biopolymers: Combining SEC with Light Scattering Detection, S.C. Kuebler, Wyatt Corporation Webinar, online at http://www.wyatt.com/, Figure 12.

(32) B.H. Zimm, J. Chem. Phys. 13(4), 141 (1945).


Ira S. Krull
Ira S. Krull is Professor Emeritus of Chemistry and Chemical Biology at Northeastern University, Boston, Massachusetts, and a member of LCGC's editorial advisory board.


Simion Kreimer
Simion Kreimer is a graduate student at Northeastern University pursuing a doctorate in the fields of chemistry and chemical biology through research in the Barnett Institute. His research interests include the application of MS and chromatography to translational medicine and pharmacology.


Anurag S. Rathore
Anurag S. Rathore is a biotech CMC consultant and an associate professor with the Department of Chemical Engineering at the Indian Institute of Delhi, India.


ADVERTISEMENT

blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:




 

LCGC COLUMNISTS 2014

Sample Prep Perspectives | Ronald E. Majors:

LCGC Columnist Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments in existing technology lines.

LATEST: The Role of Selectivity in Extractions: A Case Study

History of Chromatography | Industry Veterans:

With each installment of this column, a different industry veteran covers an aspect of the evolution and continued development of the science of chromatography, from its birth to its eventual growth into the high-powered industry we see today.

LATEST: Georges Guiochon: Separation Science Innovator

MS — The Practical Art| Kate Yu:
Kate Yu is the editor of 'MS-The Practical Art' bringing her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers.

LATEST: Mass Spectrometry for Natural Products Research: Challenges, Pitfalls, and Opportunities


LC Troubleshooting | John Dolan:

LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan, Vice President of LC Resources and world renowned expert on HPLC, is able to highlight common problems and provide remedies for them.

LATEST: LC Method Scaling, Part I: Isocratic Separations

More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>


Source: LCGC North America,
Click here