The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part I: Background, Theory, and Potential Uses - - Chromatography Online
The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part I: Background, Theory, and Potential Uses


LCGC North America
Volume 30, Issue 9, pp. 842-849

References

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(17) Protein Pegylation Application Note, Protein Solutions, Wyatt Technology, Santa Barbara, California, 1996.

(18) Pegylated Protein Modifications Application Notes, Protein Solutions, Wyatt Technology, Santa Barbara, California, 1997.

(19) T.P. Second, A.W. Carr, R.C. Cummins, R. Viner, and L. Huang, "Characterization of PEGylated Peptides and Site Localization of Attachment with High Resolution ETD Mass Spectrometry," Thermo Fisher Scientific, Fremont, California, Application Note (2010).

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(22) M.E. Szulc, R. Mhatre, J. Mazzeo, and I.S. Krull, in High Resolution Separation of Biological Macromolecules, Methods in Enzymology Series, B.L. Karger and W. Hancock, Eds. (Academic Press, Waltham, Massachusetts, 1996), Chapter 8, p. 175.

(23) C. Laue and D. Hunkeler, Int. J. Polym. Anal. Charact. 5, 511–529 (2000).

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(29) W. Schartl, Light Scattering from Polymer Solutions and Nanoparticle Dispersions (Springer-Verlag, Berlin, Germany, 2010).

(30) Absolute Characterization of Proteins and Biopolymers: Combining SEC with Light Scattering Detection, S.C. Kuebler, Wyatt Corporation Webinar, online at http://www.wyatt.com/, Figure 7.

(31) Absolute Characterization of Proteins and Biopolymers: Combining SEC with Light Scattering Detection, S.C. Kuebler, Wyatt Corporation Webinar, online at http://www.wyatt.com/, Figure 12.

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Ira S. Krull
Ira S. Krull is Professor Emeritus of Chemistry and Chemical Biology at Northeastern University, Boston, Massachusetts, and a member of LCGC's editorial advisory board.


Simion Kreimer
Simion Kreimer is a graduate student at Northeastern University pursuing a doctorate in the fields of chemistry and chemical biology through research in the Barnett Institute. His research interests include the application of MS and chromatography to translational medicine and pharmacology.


Anurag S. Rathore
Anurag S. Rathore is a biotech CMC consultant and an associate professor with the Department of Chemical Engineering at the Indian Institute of Delhi, India.


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