Trends in Pharmaceutical Analysis: A Technology Forum - - Chromatography Online
Trends in Pharmaceutical Analysis: A Technology Forum

The Column
Volume 10, Issue 9, pp. 2 to 6

Q: Are spectroscopic techniques (without chromatography) still important for pharmaceutical analysis?

AVS: The answer to this question depends on the purpose of the analysis. What type of information does the analyst aim for? A separation step might not be needed when the compound is present as the only active pharmaceutical ingredient (API) in a pharmaceutical mixture, and is simply dissolved in a simple matrix. In such cases, it may be possible to apply a stand-alone spectroscopic technique.

One example is identity testing of a bulk pharmaceutical ingredient. This is most conveniently performed by infrared (IR) spectroscopy. Indeed, the typical fingerprint region in the IR spectrum allows confirmation of the identity of a compound after comparison with the spectrum of a standard.

When the purpose of an analysis is assaying the medicine, UV testing can be a good choice. Formulations containing a single API can be conveniently analyzed with UV spectrophotometry if the excipients do not interfere in the UV absorbance. It may or may not involve a simple sample preparation procedure, and the analysis could be applied routinely because of its simplicity. It is also possible to carry out UV analysis in the form of a flow injection analysis (FIA). In FIA the samples are injected into a flowing stream of liquid that continuously passes through a detector cell. UV measurement in the cell allows very fast and automated analysis of all the samples.

When people are testing for the presence of impurities in medicines, in the majority of cases a separation technique will be implemented. In this case sample preparation may be needed for the analysis of drug products containing the formulated drug in the presence of excipients.

There are also areas in which the use of particular spectroscopic techniques (without chromatography) is emerging, such as quick and initial detection of a counterfeit drug in suspicious medicines. Raman and near infrared (NIR) spectroscopy have shown their strength in this field. These spectroscopic techniques could become as important as UV in the future.

TvW: Separation prior to detection generally results in higher specificity. However, since this is not always required, direct application of spectroscopic techniques is therefore desired in cases where timely and cost-effective analysis is paramount. Direct UV measurement is the preferred detection for dissolution testing. Vibrational spectroscopic techniques such as near infrared (NIR) and Raman are used in process control and for anti-counterfeit analysis where fast and nondestructive analyses are required. Implementation of quality-by-design (QBD) has resulted in a strong increase in the use of these techniques. In addition, spectroscopic techniques can replace visual evaluation or comparison of colour against European Pharmacopoeia reference standards, to make colour assessments more objective. These examples show that new opportunities can still be found for applying direct spectroscopic techniques.

HN: Maybe less so in small molecule pharmaceutical analysis, but in biopharmaceutical analysis, spectroscopic techniques (without chromatography) still have a very important position in product release, stability testing, or characterization. A few examples include: UV absorbance in the content analysis of protein products; absorbance or fluorescence in immune- and cell-based assays (and even in some chemical assays like those that test for free SH groups); and fluorescence and circular dichroism in the characterization of secondary and tertiary structure of proteins.

Ann Van Schepdael is a professor at the KU Leuven in Leuven, Belgium.

Tom van Wijk is a senior scientist at Abbott Healthcare BV in Weesp, the Netherlands.

Harm Niederlander was a project leader at Synthon Biopharmaceuticals in Nijmegen, the Netherlands, until August 2013.




This article is from The Column. The full issue can be found here:


blog comments powered by Disqus
LCGC E-mail Newsletters
Global E-newsletters subscribe here:



Sample Prep Perspectives | Ronald E. Majors: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments.
LATEST: UV Detector Problems

Perspectives in Modern HPLC | Michael W. Dong: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: Superficially Porous Particles: Perspectives, Practices, and Trends

MS — The Practical Art | Kate Yu: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis

LC Troubleshooting | John Dolan: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: Problems with Large-Molecule Separations

More LCGC Chromatography-Related Columnists>>

LCGC North America Editorial Advisory Board>>

LCGC Europe Editorial Advisory Board>>

LCGC Editorial Team Contacts>>

Source: The Column,
Click here