Application Notes: Pharmaceutical - Chromatography Online
Application Notes: Pharmaceutical

Polycaprolactam for controlled drug delivery - Analysis on Agilent PLgel MIXED-C

February 7, 2014

Polycaprolcatam allows for steady release of drug as the polymer degrades. Measuring molecular weight distributions of biodegradable polymers by GPC is a critical part of research into controlled drug release.

Increasing Speed for Biocharacterizations: Peptide Mapping ofMonoclonal Antibodies with an Agilent AdvanceBio Peptide Mapping Column

December 2, 2013

Agilent Technologies

Bruker EU - High Throughput Quantitative and Qualitative DMPK Measurements

September 27, 2013

This application note explains the use of quan/qual tools with fast chromatography suitable for high throughput screen of P450 microsomal metabolism of drugs in a DMPK arena.

Bruker EU - Top Down Analysis of Histone H4

September 27, 2013

Characterization of proteins by top-down mass spectrometry is often necessary when many post translational modifications are present. Histones are an example of this type of challenge due to their abundant and varying modifications. We demonstrate top-down dissociation of two intact human histone H4 proteins with ECD, ETD, CID, and MALDI-ISD on the Bruker solariX FTMS platform. These dissociation techniques were used to determine the presence of both acetylation and dimethylation on the proteins.

Bruker NA - Definitive Elemental Formula Determination Debuts with SmartFormula-HR™

September 27, 2013

In this study, SmartFormula-HR Software is applied to a mixture of pesticides. The mixture is first separated via HPLC, and then detected with high resolution mass spec?trometry using the solariX.

Bruker NA - Top Down Analysis of Histone H4

September 27, 2013

Characterization of proteins by top-down mass spectrometry is often necessary when many post translational modifications are present. Histones are an example of this type of challenge due to their abundant and varying modifications. We demonstrate top-down dissociation of two intact human histone H4 proteins with ECD, ETD, CID and MALDI-ISD on the Bruker solariX FTMS platform. These dissociation techniques were used to determine the presence of both acetylation and dimethylation on the proteins.

Thermo EU - Generic Method Approach for Pharmaceutical Drug Discovery and Development Using Reversed-Phase Hydrophilic Interaction Liquid Chromatography with Universal Charged Aerosol Detection

September 5, 2013

This generic method addresses the need to make changes during the drug development life cycle and is applicable to each new drug formulation. Using gradient reversed-phase LC in the first stage and HILIC in the second stage, both hydrophilic and hydrophobic compounds are separated in a single, short run.

Thermo EU - Intact Protein LC-MS: How to Overcome the Challenges?

September 5, 2013

LC–MS analysis of proteins can be challenging. This work describes the performance of three Thermo Scientific™ EASY-Spray™ columns dedicated to intact protein LC–MS. Recovery, separation efficiency, and resolution will be demonstrated for various conditions.

Advantages of High-Resolution Separation Media for Monoclonal Antibody Analysis

September 5, 2013

Advantages of High-Resolution Separation Media for Monoclonal Antibody Analysis

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LCGC COLUMNISTS 2014

Sample Prep Perspectives | Ronald E. Majors: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments.
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MS — The Practical Art | Kate Yu: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting | John Dolan: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: Problems with Large-Molecule Separations


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