Ron Majors, editor of "Column Watch" and "Sample Prep Perspectives," has been with LCGC North America for over 26 years. Currently a senior scientist with Agilent Technologies, Wilmington, Delaware, Ron is known industry-wide as one of the premier chromatography experts in the field. He is also a member of LCGC's editorial advisory board.
Many problems encountered executing HPLC methods are a result of decisions made during early method development. This article discusses one critical variable in method development, the choice of stationary phase chemistry.
This year's HPLC symposium was held in New Orleans, Louisiana, USA. In this review, Ron Majors covers HPLC and UHPLC column and sample preparation highlights, summarizes the awards presented, and reviews the overall liquid-phase chromatography trends.
In HPLC column development, the bonded-phase silica is often only investigated to determine the suitability of a packed column for a particular separation class and not much thought is given to optimization of the base silica to make the overall packing material more suitable for the assay. Here, the authors examine general-purpose standardized tests to determine the quality of bulk silica before and after bonding.
This year's symposium was held in New Orleans, Louisiana, USA. In this review, Ron Majors covers HPLC and UHPLC column and sample preparation highlights, summarizes the awards presented, and reviews the overall liquid-phase chromatographic trends.
Sample preparation has often been viewed as the bottleneck in analytical procedures. Surveys have shown that time is typically the most frequent problem area for sample preparation procedures. While newly developed extraction techniques address time, modern chromatography advances are also moving towards faster separations. Based on these considerations, what is high-throughput sample preparation? Do modern extraction methods adequately address the issue of time? How can we address the analytical need for speed?