Articles by Daniela Held - Chromatography Online
Articles by Daniela Held

Daniela Held


Articles
Tips & Tricks GPC/SEC: Sample Recovery and More
October 6, 2014

This article provides some tips and tricks for monitoring sample recovery and peak area using GPC/SEC.

Tips & Tricks GPC/SEC: How to Test GPC/SEC Columns
August 8, 2014

Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) columns are at the heart of a GPC/SEC system. An easy test to determine the plate count, the peak symmetry, and the resolution of the columns is therefore an essential tool to ensure GPC/SEC system functionality.

Tips & Tricks GPC/SEC: The Art of Analyzing High Molar Mass Samples
June 6, 2014

GPC/SEC can be performed for samples that range from a few hundred to several million daltons in molar mass. It can often require more skill to analyze high molar mass samples in the molar mass range of several million dalton. This article explains more.

Tips & Tricks GPC/SEC: Mobile Phase Considerations
April 8, 2014

There are many sources of advice on how to select the most appropriate stationary phase for sample analysis, but the mobile phase is not often discussed. This article provides tips for selecting the most appropriate mobile phase for your analysis.

Tips & Tricks GPC/SEC: How to Install GPC/SEC Columns
February 3, 2014

For proper column installation it is important to be familiar with column characteristics such as solvent compatibility, maximum flow rate, and maximum operating temperature.

Tips & Tricks GPC/SEC: Select the Right Columns for Your Molar Mass Range
December 11, 2013

Shoulders in gel permeation/size-exclusion chromatograms (GPC/SEC) can be a result of sample characteristics or down to the wrong choice of columns or column combinations. Proper selection helps to measure true results.

Tips & Tricks: Evaporative Light Scattering Detection in GPC/SEC
October 3, 2013

This article presents applications of ELSD in GPC/SEC. It discusses both the advantages and disadvantages as well as the practical considerations for use in GPC/SEC.

Tips & Tricks GPC/SEC:An Alternative to Calibration With Narrow Standards
August 8, 2013

Integral calibration with a broad standard is a fast, cost-effective, and easy-to-use calibration technique for quality control. This article explains more.

Tips & Tricks GPC/SEC: Analysis and Calibration for Low Molar Mass Macromolecules
June 3, 2013

GPC/SEC analysis can be easily optimized to yield improved results when analysing oligomers and low molar mass macromolecules.

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LCGC COLUMNISTS 2014

Column Watch: Ron Majors, established authority on new column technologies, keeps readers up-to-date with new sample preparation trends in all branches of chromatography and reviews developments. LATEST: When Bad Things Happen to Good Food: Applications of HPLC to Detect Food Adulteration


Perspectives in Modern HPLC: Michael W. Dong is a senior scientist in Small Molecule Drug Discovery at Genentech in South San Francisco, California. He is responsible for new technologies, automation, and supporting late-stage research projects in small molecule analytical chemistry and QC of small molecule pharmaceutical sciences. LATEST: HPLC for Characterization and Quality Control of Therapeutic Monoclonal Antibodies


MS — The Practical Art: Kate Yu brings her expertise in the field of mass spectrometry and hyphenated techniques to the pages of LCGC. In this column she examines the mass spectrometric side of coupled liquid and gas-phase systems. Troubleshooting-style articles provide readers with invaluable advice for getting the most from their mass spectrometers. LATEST: Radical Mass Spectrometry as a New Frontier for Bioanalysis


LC Troubleshooting: LC Troubleshooting sets about making HPLC methods easier to master. By covering the basics of liquid chromatography separations and instrumentation, John Dolan is able to highlight common problems and provide remedies for them. LATEST: How Much Can I Inject? Part I: Injecting in Mobile Phase


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