The parameters that should be considered in the optimization of HPLC methods are explained and then illustrated through the analysis of two commercial agricultural products.
In the 1960s, high performance liquid chromatography (HPLC) was in its infancy and Phyllis Brown was a pioneer at the forefront of the technique.
The authors set out to perform a separation of seven water-soluble vitamins without the use of ion-pair reagents.
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