Jeff Layne

Data from this technical note demonstrates the utility of the Kinetex 2.6 ?m XB-C18 column for the separation of numerous, typically difficult fluoroquinolones by LC/MS with non-ion pairing buffers.

The first and only core-shell media available for lab-scale preparative purification. Chromatographers can now scale their ultra-high efficiency Kinetex core-shell analytical columns directly to Kinetex 5?m Axia-packed preparative columns.

With virtually no development required chromatographers running traditional methods can instantly improve resolution, sensitivity, and productivity by simply replacing 5?m and 3?m fully porous columns with a Kinetex 5?m core-shell column.

Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression.

Urinary excretion profiling is a key element in the pharmokinetic characterization of new chemical entities (NCEs). Recent advances in ultra-high performance liquid chromatography (UHPLC) column technology offer improved results for liquid chromatography tandem mass spectrometry (LC–MS¬–MS) analysis, enabling rapid quantification of specific drugs and their metabolites in biological matrices.

Phenomenex

Throughout the drug development process, potential new drug candidates (or new chemical entities; NCEs) and their metabolites must be subjected to rigorous and extensive pharmacokinetic evaluations to determine their rates accumulation, metabolism, and excretion from the body. With regards specifically to the excretion, urinary excretion is typically the predominant route for the elimination of drugs and their metabolites, and urinary excretion profiling is an integral portion of the pharmacokinetic characterization of NCEs.

Phenomenex Application Note

Heterocyclic amines (HCAs) are a class of compounds that are formed when amino acids from meat proteins react with glucose and creatine under high-temperatures, as is associated with frying or grilling over an open flame (1–3). Studies have correlated the consumption of high levels of HCAs with an increased risk of some forms of cancer.