Erica Pike

Sample preparation has historically been a time-consuming challenge in high performance liquid chromatography (HPLC) and gas chromatography (GC) analysis, consisting of up to 60% of total analysis time. Automated platforms are now able to perform sample preparation techniques including solid-phase extraction (SPE) and protein precipitation. By implementing automation, a laboratory can instantly increase throughput, improve accuracy, reproducibility, and lab-to-lab consistency.

In this application note, 41 pain management drugs from several drug classifications were isolated from urine samples and analyzed by LC/MS/MS in under 5 min using a Kinetex Core-Shell technology HPLC/UHPLC column.

Eight benzodiazepines were extracted from both urine and plasma using an automated Strata-X SPE procedure and analyzed via LC/MS/MS Kinetex core-shell method.

A fast and efficient HPLC method for sensitive, accurate, and reproducible quantitation of Vitamin C in human plasma by applying Impact Protein Precipitation Plate Technology with Kinetex Core-Shell HPLC Columns.

A fast and efficient HPLC method for sensitive, accurate, and reproducible quantitation of Vitamin C in human plasma by applying Impact Protein Precipitation Plate Technology with Kinetex Core-Shell HPLC Columns.

In clinical and pharmaceutical testing, analkyses of drug compounds and their metabolites are often performed in complex biological matrices, such as plasma and whole blood.

This article examines the various effects of phospholipids in liquid chromatography tandem mass spectrometry (LC–MS-MS) analysis and demonstrates a new phospholipid-removal approach.

Phenomenex

When performing LC–MS-MS analysis, phospholipids are perhaps one of the most troublesome components of bioanalytical samples. The presence of phospholipids not only reduces the column lifetime and sensitivity but can also cause a phenomenon known as ion suppression.