Christopher J. Hudalla

Waters Corporation

How ethylene-bridged hybrid inorganic-organic (BEH) particles are prepared, and how their special quallities can be used to improve separations.

This application presents the impact that mobile phase composition (pH and ionic strength) can have on this separation of protein aggregation. It also expands on some of the primary considerations necessary for the successful transfer of a developed SE-UPLC method.

Waters Application Note

The migration to chromatographic methods utilizing smaller particle sizes has been well demonstrated to increase productivity through faster, more efficient separations.

This application demonstrates the increases in productivity attainable with conventional HPLC configurations,utilizing the new 2.5 μm, eXtended Performance (XP) columns.

How ethylene-bridged hybrid inorganic–organic (BEH) particles are prepared and how their special qualities can be used to improve separations

For successful chromatographic method development, it is advantageous to maximize the difference in column selectivity, or "selectivity space" investigated in order to minimize the number of critical analyte co-elutions.

Although supercritical fluid chromatography (SFC) is not a new technique, preparative SFC is becoming increasingly more popular with advances in instrumentation, software and chemistry.