The Application Notebook, Apr 1, 2002 - Chromatography Online
The Application Notebook, Apr 1, 2002
Environmental
Determination of Trace Cations in a Power Plant Feed Water Stabilized with 7 ppm of Monoethanolamine
Determination of zinc, lithium, cobalt, sodium, ammonium, potassium, manganese, mahnesium and calcium in power plant feed water stabilized with 7 ppm monoethanolamine using cation chromatography with direct conductivity detection...
Improved Determination of Trace Anions in High-Purity Waters by High-Volume Direct Injection with the EG40
Ion chromatography eluent generation and anion-exchange columns with with 5 µm particle size can be used to determine sub-µg/L anions in high-purity water by large-loop injection...
Proteomics
Exceptional Selectivity and Resolving Power of 300 Å Pore for Proteins and 90 Å for Peptides Mapping
The development of reproducible, ultrapure, spherical chromatographic silica with 300 Å pore size has opened the door for a new era in protein separation and purification...
General
Analysis of Polymer Branching - Triple Detection Size Exclusion Chromatography of Polycarbonates
The note describes the use of triple detection size exclusion chromatography for the analysis of polycarbonates...
Star-Branched Polymers
Star-branched polymers are materials with many fascinating properties...
Cation Exchange Separation of Proteins on a Bonded Hydrophilic Polymer-Coated Silica Support
This application note provides one example of the separation power of the BioBasic® SCX column...
Fluorescence-based Isolation of Formononetin from Red Clover Extract with the Agilent 1100 Series Purification System
This application note describes how the Agilent 1100 Series fluorescence detector is integrated into the purification ystem and how it is used to isolate formononetin from red clover extract...
Analysis of Polar Compounds using a SOLGEL-WAXTM Capillary Column
The use of sol-gel materials as stationary phases in gas chromatography is discussed in this note...
On-line MALS-QELS (Quasi-Elastic Light Scattering)
Presented is Wyatt's solution for quasi-elastic light scattering...
Medical/Biological
Haemoglobin Characterization
This note describes the use of SEC with MALS, a miniDAWN detector and an Optilab DSP interferometric refractometer for the characterization of haemoglobin...
Fast and Easy Physiological Amino Acids Analysis in 15 Minutes
Due to the complex chemical composition of urine and serum samples, the analysis of physiological amino acids has often been difficult...
2D Capillary LC-MS-MS for the Analysis of Complex Peptide Mixture
After analysing a large number of genome sequences, attention is now focused on the structure and function of proteins in biological systems...
Hyaluronic Acid (Polysaccharides)
The determination of hyaluronic acid is described...
High-Throughput Quantitaion of Midazolam in Human Serum by Ion Trap LC-MS
The note demonstrates the use of an ion-trap LC-MS-MS system for the fast, sensitive quantitation of a pharmaceutical compound in a biological matrix...
Pharmaceutical
Determination of Clozapine and Desmethylclozapine in Serum by High Throughput On-line SPE-LC-MS
In this study, a fast automated assay for CLZ, DMC and a second metabolite, clozapine N-oxide, in serum using SPE coupled on-line with LC-MS is presented...
Determination of Digoxin in Human Serum by LC-MS with On-line Sample Preparation
This application note describes a method for quantification of digoxin using LC-MS with integrated on-line sample preparation that avoids the derivatization required by other HPLC methods...
LC-MS Analysis of Ecstasy and its Metabolites in Human Urine
This application will demonstrate LC-MS methods for the rapid and straightforward determination of MDMA and metabolites in biological fluids...
Analysis of Polar lipids Using HPLC and ELS Detection
For the analysis of a polar lipid mix a method was chosen to maximize the resolution between the compounds...
Simplified Method Development for the Extraction of Acidic, Basic and Neutral Compounds
SPE has become a popular sample preparation technique that bridges the gap between specimen, collection and instrumental analysis...

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