Two promising wide-pore stationary phases were recently introduced for the fast and high-resolution separations of large biomolecules: the recently launched wide pore 3.6 ?m superficially porous particle and the hybrid-type sub-2-?m fully porous particle. This article focuses on the achievable throughput and resolution for the characterization of biomolecules.
The authors characterized twelve SFC achiral stationary phases with 60 compounds from four chemical classes to establish guidelines for the rational selection of the optimum stationary phase for separations.
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