Fully porous particle columns with narrow particle distribution can improve the efficiency of HPLC separations. In this new video from LCGC TV, Richard Henry, a consultant from State College (Pennsylvania, USA) discusses the advantages of narrow particle distribution and whether optimized LC instruments are required for optimum performance. Watch here>>
LCGC TV Video: Using the Variable Length Method Development Strategy to Develop Successful UHPLC Methods
The Kinetic Plot method is a useful tool in LC method development to compare the performance of different LC columns with each other. In this new video from LCGC TV, Deirdre Cabooter from KU Leuven (Belgium) describes how to use the variable length method development strategy to develop successful UHPLC methods and how this approach can save time for the analyst. Watch here>>
LCGC TV Video: Mary Ellen McNally on Analytical Precision
In these two new videos from LCGC TV, Mary Ellen McNally from DuPont Crop Protection addresses the issue of precision, covering the reasons why the precision of a measurement is so important and the factors that can influence the precision of an active ingredient assay.
LCGC TV Video: Why You Need to Listen . . . Even to Your Instrument
We all have always been told that we need to listen, and to listen more carefully. Growing up, we were told to listen to our parents and our teachers. As adults, we need to listen more attentively to our spouses at home and our bosses, colleagues, and customers at work. But have you considered listening to your analytical instruments? Here is why you should. Watch here>>
LCGC TV Video: Part II: Milton Lee on the Potential of Thermal Gradient Gas Chromatography (TGGC)
First developed in the 1960s to reduce peak broadening effects, thermal gradient gas chromatography (TGGC) is now being re-examined. In these three new videos from LCGC TV, Milton L. Lee from Brigham Young University discusses the application areas of TGGC, the “peak sweeping” approach, and whether or not the technique is ready for industry.
LCGC TV Video: What forensic labs are up against
Analysts in forensic labs work in a challenging landscape, where the targets of analysis keep changing. In two new short video clips, Tom Brettell explains how these challenges play out in terms of their use of GC columns and GC methods.
LCGC TV Video: Should you be using GCxGC in your routine analysis?
Two-dimensional gas chromatography (GCxGC) started out as a specialized technique reserved for the domain of academic researchers. But the technique, and the instrumentation, have evolved significantly in the past decade. Does it make sense to use GC x GC in routine analysis? Frank Dorman weighs in. Watch here>>
LCGC TV Video: How to Pick Column Combinations for GCxGC
When you are setting up a two-dimensional GC separation (GCxGC), how do you get the optimal combination of columns with the least amount of method development pain? In two new short videos, Nick Snow of Seton Hall offers advice, about column type and column length.
LCGC TV Video: Rethinking Sample Prep
When we asked Doug Raynie where he sees sample preparation heading, he replied not in terms of specific techniques, but instead in terms of the overarching considerations that we should keep in mind. Do we need to rethink sample prep? Watch here>>
LCGC TV Video: Sample Preparation for Chromatographic Methods in Metabolomics
Advances in mass spectrometry instrumentation have opened up the field of metabolomics, but there are still issues with non-standardized sampling and sample preparation methods. Tuulia Hyötyläinen from the VTT Technical Research Centre of Finland discusses the vast range of sample matrices and gives advice on best practices. Watch here>>
LCGC TV Video: Using Passive Sampling in Environmental Analysis
Anthony Gravell of Natural Resources Wales discusses the principles of passive sampling and the advantages of the technique over spot sampling when screening water samples for environmental contaminants. He also gives an overview of the challenges and considerations that have to be made when performing LC–MS on passive sampling extracts. Watch here>>
LCGC TV Video: What's Behind the Trends in Sample Preparation
There are some interesting shifts going on in sample preparation. In this new short video interview, Doug Raynie of South Dakota State University discusses what is behind the trends — starting with the fundamental differences in strategies for solid and liquid samples. Watch here>>
LCGC TV Video: Is Two-Dimensional LC Worth the Effort?
Two-dimensional liquid chromatography (2D LC) is a powerful technique, but method development is more involved and time-consuming. So for what types of analyses does it make sense to invest the extra effort to develop a 2D LC method instead of a 1D method? Dwight Stoll explains. Watch here>>
We all make mistakes. The first step to correcting them is recognizing them, and learning the right way to do things. In two new short videos, Merlin Bicking talks about two common errors that chromatographers make, and how to avoid them. Watch here>>
LCGC TV Video: The Future of Monoliths and Chips
Monoliths have received renewed attention in the past few years. Will they — or chip-based columns — ever compete with particle-based columns? In a short video interview, Dick Henry weighs in. Watch here>>
LCGC TV Video: Deirdre Cabooter on Method Development in UHPLC
Performing ultrahigh-performance liquid chromatography (UHPLC) can significantly reduce analysis time without compromising on efficiency by using sub 2-μm particles that allow increased velocity while maintaining selectivity. In this new short interview, Deirdre Cabooter of KU Leuven in Belgium, discusses the advantages of using UHPLC in method development and tips to follow when transferring methods from UHPLC to HPLC instrumentation. Watch here>>
LCGC TV Video: Two-Dimensional LC in Pharmaceutical Analysis: Advantages and Challenges
Two-dimensional liquid chromatography has moved from academia into industry. In two new short video interviews, Kelly Zhang of Genentech talks about the advantages gained by developing a two-dimensional LC method for analyzing impurities in pharmaceuticals, as well as the challenges in developing the method. Watch here>>
LCGC TV Video: GC in Forensic Laboratories
Tom Brettell, an associate professor of chemistry at Cedar Crest College, USA, recently conducted a survey of gas chromatography column use in forensic laboratories in the United States. Here is what he found, in terms of the types of columns they are using and the problems they face. Watch here>>
Thermal gradient gas chromatography (TGGC) was first developed in the 1960s to reduce the effects of peak broadening. With recent developments in technology, the technique is now being re-examined. In two new videos from LCGC TV, Milton L. Lee from Brigham Young University discusses the principles of TGGC, and the possible advantages of TGGC over traditional GC methods. Watch here>>
LCGC TV Video: Analyzing Drugs of Abuse
In a recent study, Nick Snow of Seton Hall University studied the presence of drugs of abuse in various complex matrices to detect trace quantities of drugs of abuse such as salvorinin A. In a new short video, Snow explains why used GCxGC–TOF-MS is a good choice for this type of analysis. Watch here>>
LCGC TV Video: The Pros and Cons of GCxGC
Two-dimensional gas chromatography has advanced considerably since its first emergence in the late 1980s and its first applications for the hydrocarbon fingerprinting of crude oils. In a new interview, Frank L. Dorman of Penn State University talks about the evolution of GCxGC and the key advantages and disadvantages of the technique, including why fear of its complexity gets in the way of its broader use. Watch here>>
LCGC TV Video: Eliminating Matrix Effects: LC–MS Analysis of Biological Fluids
Frederic Lynen of Ghent University in Belgium discusses why LC–MS is the ideal analytical tool for the quantitative analysis of analytes in biological samples, and presents a new HILIC-based SPE approach to improve analyte recovery and sensitivity by removing phospholipids from samples. Watch here>>
LCGC TV Video: Richard Henry on Fully Porous vs. Superficially Porous Particles
Superficially porous particles for HPLC columns – often called "core–shell" particles have gotten a lot of attention in recent years because of their excellent performance. Yet so far, columns made with fully porous particles are still the most widely used. Will superficially porous particles eventually take over? Watch here>>
In this new video interview, Dwight Stoll of Gustavus Adolphus provides advice about key decisions in method development, common errors to avoid, and other advice for getting started with 2D LC. Watch here>>
LCGC TV: Richard Henry on "Do We Need More LC Phases?
Dick Henry, consultant, discusses a trend that could renew efforts towards developing novel LC stationary phases with improved selectivity, peak symmetry, and stability. The question is, however, whether we really need more stationary phases in HPLC. Watch the video and find out. Watch here>>
LCGC TV: Mary Ellen McNally on Common Error with Internal Standard Methods
Internal standards are an important tool of analytical chemists, yet errors in their usage are common. Mary Ellen McNally of DuPont Crop Protection offers advice for how to introduce an internal standard and how to avoid common errors with internal standard methods. Watch here>>
LCGC TV: Michal Holcapek on Trends in LC–MS
More and more analysts are moving away from low-resolution mass spectrometry to high-resolution mass spectrometry, but what are the requirements for making such a change? Michal Holcapek, professor of analytical chemistry at the University of Pardubice, Czech Republic, discusses what you need to consider when switching to a high resolution system. Holcapek also offers advice on how to choose the most effective ionization technique for your analysis. Watch here>>
LCGC TV: Koen Sandra on Analyzing Protein Biopharmaceuticals Using LC–MS
By 2020, more than 50% of approved drugs are expected to be protein biopharmaceuticals. Koen Sandra from the Research Institute for Chromatography in Belgium discusses the challenges of characterizing protein biopharmaceuticals and why LC–MS is now indispensable for characterizing this class of drugs. Watch here>>
LCGC TV: Joe Foley on Sequential Elution Liquid Chromatography
Sequential-elution LC is a new methodology for the analysis of complex samples. In a new video initiative from LCGC, Joe Foley, Professor of Chemistry at Drexel University, Philadelphia, Pennsylvania, USA, explains very briefly how it works and its advantages over conducting multiple runs of conventional LC. Watch here>>