Liquid Chromatography (LC/HPLC)

May 19, 2016
The Column
A simple, rapid, and robust ultrahigh-performance liquid chromatography (UHPLC) method has been developed for the simultaneous determination of natural and artificial vanilla flavouring substances as well as some precursors has been developed using an automated method scouting or method optimization workflow.
May 01, 2016
LCGC North America
How would you analyze a bag of gummy bears that showed up on your laboratory bench? This was the challenge taken on by teams of analysts in advance of the Conference on Small Molecule Science (CoSMoS) that was held in August 2015 in San Diego, California. This article shares insights from how the finalists approached the question.
Apr 26, 2016
The Column
This article considers some of the current issues for analysis of small and large molecules, and emerging trends in method development.
Apr 19, 2016
LCGC Europe
Appropriate analytical methods are required to evaluate the presence, metabolism, degradation, and removal of specific compounds in complex mixtures. There is an increasing demand to analyze samples with a wide range of polarities in a variety of applications, including environmental analysis, biomarker discovery, and proteomics. Multiple analyses on complementary columns are often needed to cover the separation of all compounds with a large difference in polarity. This article describes a generic method involving an ultrahigh-pressure liquid chromatography (UHPLC) system equipped with two external switching valves to connect hydrophilic interaction liquid chromatography (HILIC) and reversed-phase LC columns in series for the sequential analysis of polar and apolar compounds. The method was successfully applied to separate 32 pharmaceutical compounds with a wide range of polarities, which could be useful for analyzing pharmaceutical compounds in the environment.
Apr 18, 2016
As a result of advances in multilevel state-of-the-art mass spectrometry (MS) methods, combined with chromatographic and electrophoretic techniques, very precise characterization of biotherapeutics such as monoclonal antibodies (mAbs) and antibody drug conjugates (ADCs) is now possible. Until recently, however, these techniques were considered suitable only for research use. With the advent of robust and user-friendly solutions, these techniques are now amenable for routine use, as illustrated by examples of applications of the characterization of mAbs and ADCs. This is the fourth in a series of four articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24.
Apr 08, 2016
Until recently, mass spectrometry (MS) was limited in the information it could supply regarding proteins larger than 40 kDa. The most recent instruments have broken through that limit, but proteins smaller than 40 kDa are still more easily detected in MS and can suppress the collection of data from larger proteins. This situation has created a demand for better separation of proteins upstream from the MS orifice. At present, though, this separation of proteins is something of a bottleneck. Methods such as reversed-phase chromatography that involve mobile phases compatible with MS are not compatible with many proteins. Alternative modes of chromatography include size-exclusion chromatography, ion-exchange chromatography, hydrophilic interaction chromatography (HILIC), and hydrophobic interaction chromatography (HIC). We decided to take another look at HIC. This is the third in a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24.
Apr 04, 2016
E-Separation Solutions
Separations of intact proteins play many roles in drug discovery and development. A variety of separation techniques are used, from immunoprecipitation for study of a single protein of interest, through various types of column chromatography for detecting a handful of proteins at once, all the way to proteomics for studying hundreds to thousands of proteins. What all of these techniques and applications have in common is that the power of protein separations is limited by the fact that proteins are large, slowly diffusing, sticky molecules. This article discusses various chromatographic approaches to addressing this challenge. This is the second in a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24.
Apr 01, 2016
LCGC North America
Our annual review of new LC columns and accessories introduced at Pittcon and through the previous year.
Apr 01, 2016
LCGC North America
Our annual review of new HPLC instruments and related products highlights the innovative features of new HPLC systems, modules, software, and product extensions.
Apr 01, 2016
Special Issues
When selecting the optimum phase for SEC separations, several key column parameters must be considered carefully.
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