Liquid Chromatography (LC/HPLC)

Apr 01, 2016
LCGC North America
What should you do if your retention times drop from one injection to the next?
Mar 14, 2016
In proteomics studies, proteins are digested into hundreds of thousands of peptides, thus creating very large and complicated mixtures. Simultaneous electrospray ionization of these complex mixtures, however, results in suppression of ion formation. Therefore, it is essential to have effective chromatographic methods to separate the peptides before analysis with mass spectrometry, to relieve ion suppression and to allow the mass spectrometer sufficient time to collect tandem mass spectra of peptide ions. The challenges involved in developing such separations are great, however. This is the first of a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24.
Mar 01, 2016
LCGC North America
How suitable is the column plate number for system suitability testing?
Feb 01, 2016
LCGC North America
Pavel Jandera discusses building his own LC instrument, the birth of modern HPLC, the importance of exploring (and understanding) earlier research papers, and current trends in contemporary chromatography. He also offers inspiring advice for young chromatographers.
Feb 01, 2016
LCGC North America
This exploration of the fundamental interactions involved in PFP stationary phases will facilitate the choice of appropriate stationary phases and help analysts develop more robust and reliable methods.
Feb 01, 2016
LCGC North America
The silica-based packing in reversed-phase columns is not inert. Here we consider what happens when the mobile phase pH is too high or too low.
Jan 01, 2016
LCGC North America
By LCGC Editors
Affinity separations offer great potential for pharmaceutical analysis.
Jan 01, 2016
LCGC North America
An investigation into the root cause of new peaks revealed an unusual source of contamination.
Jan 01, 2016
LCGC North America
Best practices and recent advances in characterizing glycosylation
Nov 01, 2015
Special Issues
This article provided guidance for working with the low-dispersion, small-volume columns that were gaining popularity in 2003. These considerations are still appropriate today with the short, narrow HPLC and UHPLC columns now in vogue. Anatomy
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