Liquid Chromatography (LC/HPLC)

Apr 08, 2016
Until recently, mass spectrometry (MS) was limited in the information it could supply regarding proteins larger than 40 kDa. The most recent instruments have broken through that limit, but proteins smaller than 40 kDa are still more easily detected in MS and can suppress the collection of data from larger proteins. This situation has created a demand for better separation of proteins upstream from the MS orifice. At present, though, this separation of proteins is something of a bottleneck. Methods such as reversed-phase chromatography that involve mobile phases compatible with MS are not compatible with many proteins. Alternative modes of chromatography include size-exclusion chromatography, ion-exchange chromatography, hydrophilic interaction chromatography (HILIC), and hydrophobic interaction chromatography (HIC). We decided to take another look at HIC. This is the third in a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24.
Apr 04, 2016
E-Separation Solutions
Separations of intact proteins play many roles in drug discovery and development. A variety of separation techniques are used, from immunoprecipitation for study of a single protein of interest, through various types of column chromatography for detecting a handful of proteins at once, all the way to proteomics for studying hundreds to thousands of proteins. What all of these techniques and applications have in common is that the power of protein separations is limited by the fact that proteins are large, slowly diffusing, sticky molecules. This article discusses various chromatographic approaches to addressing this challenge. This is the second in a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24.
Apr 01, 2016
LCGC North America
Our annual review of new LC columns and accessories introduced at Pittcon and through the previous year.
Apr 01, 2016
LCGC North America
Our annual review of new HPLC instruments and related products highlights the innovative features of new HPLC systems, modules, software, and product extensions.
Apr 01, 2016
Special Issues
When selecting the optimum phase for SEC separations, several key column parameters must be considered carefully.
Apr 01, 2016
Special Issues
Recent advances in chiral stationary phases have enabled higher efficiency and faster separations in studies of the differing enantiomeric activity of pesticides, their environmental transformation, and the degradation of pollutants in general.
Apr 01, 2016
Special Issues
When presented with a new analyte, a bioanalytical CRO must quickly develop a robust method with good chromatographic resolution, repeatable results, and a quick run time. Recent developments in LC column technology make that possible.
Apr 01, 2016
Special Issues
An investigation of C18 and phenyl-hexyl column chemistries for definitive identification of 13 synthetic cannabinoid metabolites in patient samples.
Apr 01, 2016
LCGC North America
What should you do if your retention times drop from one injection to the next?
Mar 14, 2016
In proteomics studies, proteins are digested into hundreds of thousands of peptides, thus creating very large and complicated mixtures. Simultaneous electrospray ionization of these complex mixtures, however, results in suppression of ion formation. Therefore, it is essential to have effective chromatographic methods to separate the peptides before analysis with mass spectrometry, to relieve ion suppression and to allow the mass spectrometer sufficient time to collect tandem mass spectra of peptide ions. The challenges involved in developing such separations are great, however. This is the first of a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24.
lorem ipsum